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A scaled-up Seinhorst elutriator for extraction of cyst nematodes from soil

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For more content, see Nematologica.

In order to process large soil samples containing potato cyst nematodes, the Seinhorst (1964) cyst elutriator was scaled up to process both sandy and marine-clay soils in batches of up to 2.5 kg. Several modifications were implemented. To maintain the required upward current of 3.01 min−1, an inflow of 8.01 min−1 was necessary in the enlarged, 7.5 cm diam. sedimentation tube. Also water inflow is now regulated using a flow meter with pressuriser. Several experiments were undertaken, using artificial sandy soil and marine-clay soil, both naturally infested with potato cyst nematodes. In the final experiment, using the 8.01 min−1 inflow for 4 min, there was a loss of 0.65% and 0.74% of cysts, and eggs and juveniles, respectively. This indicates that the yield (99%) of the scaled-up elutriator matches that of the original Seinhorst elutriator and, therefore, is suitable for statutory and scientific research.

Affiliations: 1: Plant Research International, PB 16, 6700 AA Wageningen, The Netherlands; 2: Applied Plant Research, Edelhertweg 1, 8219 PH Lelystad, The Netherlands

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/content/journals/10.1163/156854107781351990
2007-05-01
2016-12-06

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