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Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers

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For more content, see Nematologica.

A single randomly amplified polymorphic DNA (RAPD) marker, OPA06477, species-specific to the soybean cyst nematode species, Heterodera glycines, was identified. After sequencing the RAPD-PCR products, primers of 24 nucleotides were designed to complement the terminal DNA sequence of the DNA fragments. This resulted in one pair of species-specific primers that were used to amplify the sequence characterised amplified regions (SCAR). The developed sets of SCAR primers were successfully used in straightforward, fast and reliable PCR assays to identify H. glycines. The SCAR markers can be amplified from DNA from single second-stage juveniles and females. This is the first time SCAR primers have been combined with universal primers D2A and D3B in order to avoid false negative results.

Affiliations: 1: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China, Jilin Agricultural University, Changchun, Jilin 130118, China; 2: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;, Email: dlpeng@ippcaas.cn; 3: Jilin Agricultural University, Changchun, Jilin 130118, China; 4: Institute for Agricultural and Fisheries Research, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Ghent, Belgium

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/content/journals/10.1163/156854108783900212
2008-05-01
2016-12-06

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