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Nutritional substances for mutualistic symbiosis between Busaphelenchus xylophilus and its associated bacterium, Pseudomonas fluorescens GcM5-1A isolate

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Crude extracts of the surface coat (SC) proteins of the pine wood nematode (PWN), Bursaphelenchus xylophilus, and purified flagellin from Pseudomonas fluorescens (GcM5-1A) isolate were bioassayed using symbiontic GcM5-1A and axenic PWNs, respectively. The results showed that the flagellin significantly increased the reproduction of axenic PWN and the SC protein extracts of the PWN promoted GcM5-1A multiplication. The finding is a new step to further elucidate the symbiotic mechanism between the PWN and its associated bacteria and the SC protein function in pine wilt disease.

Affiliations: 1: 1Department of Forest Protection, Nanjing Forestry University, Nanjing, P.R. China; 2: 2Department of Biology, Qingdao University, Qingdao, P.R. China


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