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Steinernema sacchari n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa

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A new species of entomopathogenic nematode, Steinernema sacchari n. sp., was isolated by trapping with the sugar cane borer, Eldana saccharina, from soil of a sugar cane field in the KwaZulu-Natal province of South Africa. The new species is morphologically characterised by the length of the infective juvenile (IJ) of 680 (630-722) μm, tail length of 64 (51-74) μm, ratio a = 19 (14-23), H% = 49 (43-57) and E% = 82 (70-109). The pattern of the lateral field of the IJ of the new species is 2, 5, 2 ridges (3, 6, 3 lines or incisures). The male of the first generation can be recognised by the long spicule of 83 (73-89) μm, gubernaculum of 61 (50-68) μm, D% = 67 (54-88) and GS% = 73 (66-81). The first generation male lacks a mucron, while the second generation male always has one. The first generation female can be recognised by the vulval lips not being raised, the possession of long double-flapped epiptygmata and the lack of a postanal swelling. Analysis of the ITS and D2D3 regions showed S. sacchari n. sp. to differ from all other Steinernema species and to belong to a new monophyletic group, the ‘Cameroonian’ clade, consisting of S. cameroonense, S. nyetense and S. sacchari n. sp. This group is closely related to the feltiae-kraussei-oregonense Clade III.

Affiliations: 1: 1Department of Biology, Nematology Research Group, University of Ghent, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium; 2: 2Directorate Inspection Services, Department of Agriculture, Forestry and Fisheries, Private Bag X5015, Stellenbosch, 7599, South Africa; 3: 3South African Sugar Research Institute, Private Bag X02, Mount Edgecombe 4300, South Africa; 4: 4Laboratory for Electron Microscopy, North-West University, Potchefstroom Campus, Private Bag X6001, Potchefstroom 2520, South Africa; 5: 5Department of Conservation Ecology and Entomology, Department of AgriSciences, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa

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2014-01-01
2018-07-22

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