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Description of Bursaphelenchus piceaesp. n. (Nematoda: Parasitaphelenchinae) from larval galleries of the six-toothed spruce bark beetle, Pityogenes chalcographus(L.) (Coleoptera: Curculionidae: Scolytinae), in Norway spruce, Picea abies (L.) Karsten

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Bursaphelenchus piceaesp. n. is described from the bark of Norway spruce, Picea abies. Adults and propagative juveniles of the nematode are found in larval galleries of the six-toothed spruce bark beetle, Pityogenes chalcographus, while its dauer juveniles aggregate in Malpighian tubules of older larvae, pupae and adult beetles. The new species is characterised by the body length of 719 (530-945) μm in female and 632 (474-808) μm in male, its moderately slender body (a = 41.4 (35.4-50.2) and 42.6 (35.1-47.2) in female and male, respectively), and small spicules (16.0 (14.0-18.3) μm along arc and 13.7 (12.2-15.5) μm along chord). The extended anterior vulval lip in female, lateral fields with four incisures, long post-uterine sac extending for 67-91% of vulva-anus distance, and number (7) and arrangement of male caudal papillae suggest that B. piceaesp. n. is closely related to the xylophilus-group yet it differs by the relatively small, claw-like spicules with no cucullus at the tip. The close relation of B. piceaesp. n. to the xylophilus-group has been confirmed by DNA sequencing and phylogenetic analysis. Morphologically B. piceaesp. n. most closely resembles B. tokyoensisand B. fagibut can be separated from both by the unique shape of the spicules. The taxonomic separation of the new species is also confirmed by the unique molecular profile of the ITS region (ITS-RFLP). The presence of dauer juveniles of B. piceaesp. n. in Malpighian tubules of adult P. chalcographusmay lead to extensive damage to this organ as shown by local expansion of the tubule basement membrane and degradation of its cellular epithelium. Detailed phylogenetic analysis revealed that B. piceaesp. n. together with five other bark beetle-associated Bursaphelenchusspecies, namely B. trypophloei, B. masseyi, B. tiliae, B. tokyoensisand B. fagi, constitutes a small phylogenetic clade that is most closely located to, but separate from, the xylophilus-group.

Affiliations: 1: 1Department of Biological Pest Control, Institute of Plant Protection, Wladyslawa Węgorka 20, 60-318 Poznan, Poland; 2: 2Museum and Institute of Zoology, Polish Academy of Sciences, Wilcza 64, 00-679 Warsaw, Poland


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