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A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil

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Early detection and quantification of Meloidogyne hapla in soil is essential for effective disease management. The purpose of this study was to develop a real-time PCR assay for detection of M. hapla in soil. Primers and a TaqMan probe were designed for M. hapla detection. The assay detected M. hapla and showed no significant amplification of DNA from non-target nematodes. The assay was able to detect M. hapla in a background of plant and soil DNA. A dilution series of M. hapla eggs in soil showed a high correlation ( R 2 = 0 . 95 , P < 0 . 001 ) with Ct values. The assay could predict root-knot development in carrots by testing soils before planting. The assay could be useful for management decisions in carrot cultivation.

Affiliations: 1: 1Department of Agroecology, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark; 2: 2Nematology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA


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