Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Hoplotylus femina s’Jacob, 1960 (Nematoda: Pratylenchidae) from Spain with molecular phylogenetic relationships inferred by D2-D3 expansion fragments of 28S and the partial 18S rRNA gene sequences

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Nematology
For more content, see Nematologica.

This is the first report of Hoplotylus femina in Spain. It was collected from the rhizosphere and roots of common alder (Alnus glutinosa) in La Alberca, Salamanca Province, in Spain. The morphology and morphometrics of the Spanish population of H. femina agree closely with the original description of the species and other descriptions from The Netherlands, USA, New Zealand, Poland, Japan and Mexico. Only small differences in some morphological characters were found probably due to geographical intraspecific variability and/or different methods of fixing nematodes. This study provides new molecular markers for species identification (D2-D3 expansion regions of 28S and ITS-rRNA genes) and a new phylogenetic position for the D2-D3 region marker. Maximum likelihood analysis using the Shimodaira-Hasegawa test did not support the inclusion of Radopholus and Hoplotylus in the Pratylenchidae and both genera were more related phylogenetically to Hoplolaimidae.

Affiliations: 1: Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain


Full text loading...


Data & Media loading...

1. Baldwin J.G. , Luc M. , Bell A.H. (1983). "Contribution to the study of the genus Pratylenchoides Winslow (Nematoda: Tylenchida)". Revue de Nématologie Vol 6, 111-125.
2. Bernard E.C. , Niblack T.L. (1982). "Review of Hoplotylus s’Jacob (Nematoda: Pratylenchidae)". Nematologica Vol 28, 101-109.
3. Bert W. , Leliaert F. , Vierstraete A.R. , Vanfleteren J.R. , Borgonie G. (2008). "Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nematoda: Rhabditida)". Molecular Phylogenetics and Evolution Vol 48, 728-744.
4. Brzeski M. (1985). "Contribution to the knowledge of Polish plant parasitic nematodes (Nematoda). 7. Ten species new for Polish fauna". Fragmenta Faunistica Vol 29, 275-288.
5. Castillo P. , Vovlas N. (2007). Pratylenchus (Nematoda: Pratylenchidae): diagnosis, biology, pathogenicity and management. Nematology Monographs & Perspectives 6 (Series Editors: Hunt D.J. , Perry R.N. ). Leiden, The Netherlands, Brill.
6. Castillo P. , Vovlas N. , Subbotin S.A. , Troccoli A. (2003). "A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in Southern Spain". Phytopathology Vol 93, 1093-1102.
7. Coolen W.A. (1979). "Methods for extraction of Meloidogyne spp. and other nematodes from roots and soil". In: Lamberti F. , Taylor C.E. (Eds). Root-knot nematodes (Meloidogyne species). Systematics, biology and control. New York, NY, USA, Academic Press, pp.  317-329.
8. Darriba D. , Taboada G.L. , Doallo R. , Posada D. (2012). "jModelTest 2: more models, new heuristics and parallel computing". Nature Methods Vol 9, 772.
9. De Grisse A.T. (1969). "Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires". Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit Gent Vol 34, 351-369.
10. De Ley P. , Félix M.A. , Frisse L.M. , Nadler S.A. , Sternberg P.W. , Thomas K.W. (1999). "Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae)". Nematology Vol 1, 591-612.
11. Franco-Navarro F. , Cid del Prado-Vera I. (2006). "Descripción de una población mexicana de Hoplotylus femina s’Jacob, 1960 asociada a Quercus crassipes Humb. & Bonpl." Nematropica Vol 36, 173-180.
12. Ghaderi R. , Karegar A. , Niknam G. , Subbotin S.A. (2014). "Phylogenetic relationships of Telotylenchidae Siddiqi, 1960 and Merliniidae Siddiqi, 1971 (Nematoda: Tylenchida) from Iran, as inferred from the analysis of the D2-D3 expansion fragments of 28S rRNA gene sequences". Nematology Vol 16, 863-877.
13. Hall T.A. (1999). "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT". Nucleic Acids Symposium Series Vol 41, 95-98.
14. Holterman M. , van der Wurff A. , van den Elsen S. , van Megen H. (2006). "Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades". Molecular Biology and Evolution Vol 13, 1792-1800.
15. Joyce S.A. , Burnell A.M. , Powers T.O. (1994). "Characterization of Heterorhabditis isolates by PCR amplification of segments of mtDNA and rDNA genes". Journal of Nematology Vol 26, 260-270.
16. Katoh K. , Standley D.M. (2013). "MAFFT multiple sequence alignment 542 software version 7: improvements in performance and usability". Molecular Biology and Evolution Vol 30, 772-780.
17. Luc M. (1987). "A reappraisal of Tylenchina (Nemata). 7. The family Pratylenchidae Thorne, 1949". Revue de Nématologie Vol 10, 203-218.
18. Minagawa N. (1988). "Morphology of Hoplotylus femina and H. silvaticus (Nematoda: Tylenchida) from Japan". Bulletin of the National Institute of Agro-Environmental Sciences Vol 5, 175-184.
19. Page R.D.M. (1996). "TREEVIEW: an application to display phylogenetic trees on personal computers". Computer Applications in the Biosciences Vol 12, 357-358.
20. Palomares-Rius J.E. , Castillo P. , Liébanas G. , Vovlas N. , Landa B.B. , Navas-Cortés J.A. , Subbotin S.A. (2010). "Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae". Nematology Vol 12, 429-451.
21. Palomares-Rius J. , Guesmi I. , Horrigue-Raouani N. , Cantalapiedra-Navarrete C. , Liébanas G. , Castillo P. (2014). "Morphological and molecular characterisation of Pratylenchus oleae n. sp. (Nematoda: Pratylenchidae) parasitizing wild and cultivated olives in Spain and Tunisia". European Journal of Plant Pathology Vol 140, 53-67.
22. Ronquist F. , Huelsenbeck J.P. (2003). "MrBAYES3: Bayesian phylogenetic inference under mixed models". Bioinformatics Vol 19, 1572-1574.
23. Rybarczyk-Mydłowska K. , van Megen H. , van den Elsen S. , Mooyman P. , Karssen G. , Bakker J. , Helder J. (2014). "Both SSU rDNA and RNA polymerase II data recognise that root-knot nematodes arose from migratory Pratylenchidae, but probably not from one of the economically high-impact lesion nematodes". Nematology Vol 16, 125-136.
24. Ryss A.Y. (1988). Kornevye paraziticheskie nematody semeistva Pratylenchidctr (Tylenchida) mirovoi fauny. [Root parasitic nematodes of the family Pratylenchidae (Tylenchida) of the world fauna.] Leningrad, USSR, Nauka.
25. Shimodaira H. , Hasegawa M. (1999). "Multiple comparisons of log-likelihoods with applications to phylogenetic inference". Molecular Biology and Evolution Vol 16, 1114-1116.
26. Siddiqi M.R. (2000). Tylenchida parasites of plants and insects, 2nd edition. Wallingford, UK, CABI Publishing.
27. s’Jacob J.J. (1960). " Hoplotylus femina n. g., n. sp. (Pratylenchinae: Tylenchida) associated with ornamental trees". Nematologica Vol 4, 317-321.
28. s’Jacob J.J. (1979). "The description of the male of Hoplotylus, collected from oak forest". Nematologica Vol 25, 147-148.
29. Sturhan D. (2008). "Review of the genera Hoplotylus and Radopholoides (Tylenchida, Pratylenchidae)". Journal of Nematode Morphology and Systematics Vol 11, 159-166.
30. Sturhan D. (2012). "Contribution to a revision of the family Merliniidae Ryss, 1998, with proposal of Pratylenchoidinae subfam. n., Paramerlinius gen. n., Macrotylenchus gen. n. and description of M. hylophilus sp. n. (Tylenchida)". Journal of Nematode Morphology and Systematics Vol 15, 127-147.
31. Sturhan D. , Lisková M. (2007). "Notes on morphology, distribution and ecology of Hoplotylus femina (Tylenchida, Pratylenchidae)". Nematologia Mediterranea Vol 35, 243-246.
32. Subbotin S.A. , Sturhan D. , Chizhov V. , Vovlas N. , Baldwin J. (2006). "Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences". Nematology Vol 8, 455-474.
33. Subbotin S.A. , Ragsdale E.J. , Mullens T. , Roberts P.A. , Mundo-Ocampo M. , Baldwin J.G. (2008). "A phylogenetic framework for root-lesion nematodes of the genus Pratylenchus (Nematoda): evidence from 18S and D2-D3 expansion segments of 28S ribosomal RNA genes and morphological characters". Molecular Phylogenetics and Evolution Vol 48, 491-505.
34. Swofford D.L. (2003). PAUP∗. Phylogenetic analysis using parsimony (∗and other methods), version 4.0b 10. Sunderland, MA, USA, Sinauer Associates.
35. Trinh P.Q. , Waeyenberge L. , Nguyen C.N. , Baldwin J.G. , Karssen G. , Moens M. (2009). " Apratylenchus vietnamensis gen. n., sp. n. and A. binhi gen. n., sp. n., sedentary Pratylenchidae (Nematoda: Tylenchida) from coffee in Vietnam, with proposal of Apratylenchinae subfam. n." Nematology Vol 11, 565-581.
36. van Megen H. , van den Elsen S. , Holterman M. , Karssen G. , Mooyman P. , Bongers T. , Holovachov O. , Bakker J. , Helder J. (2009). "A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences". Nematology Vol 11, 927-950.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Nematology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation