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Transfer of Bursaphelenchus okinawaensis (Nematoda: Aphelenchoididae) associated with Monochamus maruokai (Coleoptera: Cerambycidae) into M. alternatus (Coleoptera: Cerambycidae) and Psacothea hilaris (Coleoptera: Cerambycidae)

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We examined the transfer of Bursaphelenchus okinawaensis associated with Monochamus maruokai into M. alternatus and Psacothea hilaris using a simple nematode loading method in order: i) to clarify the effects of cerambycid beetles on the formation of dauer juveniles of B. okinawaensis; and ii) to clarify whether dauer juveniles transfer to cerambycid beetles other than M. maruokai. Dauer juveniles appeared at a high percentage without M. alternatus or P. hilaris and these beetles did not have any positive effects on their formation. Dauer juveniles transferred to the adults of M. alternatus and P. hilaris. We concluded that dauer juveniles of B. okinawaensis form readily without its vector beetles and may be able to transfer to many kinds of cerambycid beetles, although the numbers carried by a beetle are small.

Affiliations: 1: 1Tohoku Research Center, Forestry and Forest Products Research Institute, 92-25 Nabeyashiki, Shimo-Kuriyagawa, Morioka, Iwate 020-0123, Japan ; 2: 2Forest Pathology Laboratory, Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan

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