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Genetic diversity of Meloidogyne spp. parasitising potato in Brazil and aggressiveness of M. javanica populations on susceptible cultivars

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Root-knot nematodes (Meloidogyne spp.) significantly impact potato production worldwide and in Brazil they are considered one of the most important group of nematodes affecting potatoes. The objectives of this study were to survey Meloidogyne spp. associated with potatoes in Brazil, determine their genetic diversity and assess the aggressiveness of M. javanica on two susceptible potato cultivars. Fifty-seven root-knot nematode populations were identified using esterase phenotyping, including Meloidogyne javanica, M. incognita, M. arenaria and M. ethiopica. Overall, root-knot nematodes were present in ca 43% of sampled sites, in which M. javanica was the most prevalent species, and the phenotypes Est J3, J2a and J2 occurred in 91.2, 6.7 and 2.1% of the positive samples, respectively. Other species, such as M. incognita, M. arenaria and M. ethiopica, were found less frequently and occurred at rates of 6.4, 4.3 and 2.1% of the samples, respectively. Sometimes, M. javanica was found in mixtures with other root-knot nematodes in ca 10.6% of sites containing Meloidogyne. After confirming the identification of 17 isolates of M. javanica and one isolate each of M. incognita, M. arenaria and M. ethiopica by SCAR markers, the populations were used to infer their genetic diversity using RAPD markers. Results revealed low intraspecifc genetic diversity among isolates (13.9%) for M. javanica. Similarly, M. javanica sub-populations (J2a) clustered together (81% of bootstrap), indicating subtle variation from typical J3 populations. The aggressiveness of four populations of M. javanica from different Brazilian states on two susceptible potato cultivars was tested under glasshouse conditions. Results indicated differences in aggressiveness among these populations and showed that potato disease was proportional to nematode reproduction factor.

Affiliations: 1: 1Universidade Federal de Pelotas, CP 354, Pelotas, RS 96010-900, Brazil ; 2: 2Embrapa – Clima Temperado, CP 403, Pelotas, RS 96010-971, Brazil ; 3: 3Embrapa Recursos Genéticos e Biotecnologia, CP 02372, Brasília, DF 70849-970, Brazil ; 4: 4INRA-UMR 1355, UNS, CNRS-UMR 7254, Institut Sophia Agrobiotech, 400 route des Chappes BP167, 06903 Sophia Antipolis, France

*Corresponding author, e-mail: regina.carneiro@embrapa.br
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1. Barker K.R., Koenning R. (1998). "Developing sustainable systems for nematode management". Annual Review of Phytopathology Vol 36, 165-205. [Crossref]
2. Barker K.R., Todd F.A., Shane W.W., Nelson L.A. (1981). "Interrelationships of Meloidogyne species with flue-cured tobacco". Journal of Nematology Vol 13, 67-79.
3. Baum T.J., Gresshoff P.M., Lewis S.A., Dean R.A. (1994). "Characterization and phylogenetic analysis of 4 root-knot nematode species using DNA amplification fingerprinting and automated polyacrylamide-gel electrophoresis". Molecular Plant-Microbe Interactions Vol 7, 39-47. [Crossref]
4. Brodie B.B., Evans K., Franco J. (1993). "Nematode parasites of potatoes". In: Evans K., Trudgill D.L., Webster J.M. (Eds). Plant parasitic nematodes in temperate agriculture. Wallingford, UK, CAB International, pp.  87-132.
5. Carneiro R.M.D.G., Almeida M.R.A. (2001). "Técnica de eletroforese usada no estudo de enzimas dos nematoides de galhas para identificação de espécies". Nematologia Brasileira Vol 25, 555-560.
6. Carneiro R.M.D.G., Almeida M.R.A., Carneiro R.G. (1996). "Enzyme phenotypes of Brazilian isolates of Meloidogyne spp." Fundamental and Applied Nematology Vol 19, 555-560.
7. Carneiro R.M.D.G., Castagnone-Sereno P., Dickson D.W. (1998). "Variability among isolates of Meloidogyne javanica of Brazil". Fundamental and Applied Nematology Vol 21, 319-326.
8. Carneiro R.M.D.G., Almeida M.R.A., Quénehérvé P. (2000). "Enzyme phenotypes of Meloidogyne spp. populations". Nematology Vol 2, 645-654. [Crossref]
9. Carneiro R.M.D.G., Gomes C.B., Almeida M.R.A., Gomes A.C.M.M., Martins I. (2003). "Primeiro registro de Meloidogyne ethiopica Whitehead, 1968, em plantas de quivi no Brasil e reação em diferentes plantas cultivadas". Nematologia Brasileira Vol 27, 151-158.
10. Carneiro R.M.D.G., Tigano M.S., Almeida M.R.A., Sarah J.L. (2004). "Identification and genetic diversity of Meloidogyne spp. (Tylenchida: Meloidogynidae) on coffee from Brazil, Central America and Hawaii". Nematology Vol 6, 287-298. [Crossref]
11. Carpenter A.S., Lewis S.A. (1991). "Agressiveness and reproduction of four Meloidogyne arenaria populations on soybean". Journal of Nematology Vol 23, 232-238.
12. Castro J.M.C., Lima R., Carneiro R.M.D.G. (2003). "Variabilidade isoenzimática de populações de Meloidogyne spp. proveniente de regiões brasileiras produtoras de soja". Nematologia Brasileira Vol 27, 1-12.
13. Charchar J.M. (1997). "Nematoides associados a cultura da batata (Solanum tuberosum L.) nas principais regiões de produção do Brasil". Nematologia Brasileira Vol 21, 49-60.
14. Charchar J.M., Moita A.W. (2001). "Resistência de genótipos de batata a Meloidogyne javanica". Pesquisa Agropecuária Brasileira Vol 36, 535-540. [Crossref]
15. Charchar J.M., Gonzaga V., Vieira J.V., Oliveira V.R., Moita A.W. (2007). "Efeito de nematicidas fumigantes e não fumigantes no controle de Meloidogyne spp. em batata e cenoura". Nematologia Brasileira Vol 31, 59-66.
16. Cofcewicz E.T., Carneiro R.M.D.G., Castagnone-Sereno P., Quénéhervé P. (2004). "Enzyme phenotypes and genetic diversity of root-knot nematodes parasitising Musa in Brazil". Nematology Vol 6, 85-95. [Crossref]
17. Correa V.R., Mattos V.S., Almeida M.R.A., Santos M.F.A., Tigano M.S., Castagnone-Sereno P., Carneiro R.M.D.G. (2013). "Genetic diversity of the root-knot nematode Meloidogyne ethiopica and development of a species-specific SCAR marker for its diagnosis". Plant Pathology Vol 63, 476-483. [Crossref]
18. Esbenshade P.R., Triantaphyllou A.C. (1985). "Use of enzyme phenotypes for the identification of Meloidogyne species". Journal of Nematology Vol 17, 6-20.
19. Gomes C.B. (2010). "Tubérculo livre de nematoides vale mais". A Granja Vol 71, 58-60.
20. Gomes C.B., Souza R.M. (2003). "Doenças causadas por nematoides". In: Pereira A.S., Daniels J. (Eds). O cultivo da batata na região sul do Brasil. Brasília, Brazil, Embrapa, pp.  321-349.
21. Hussey R.S., Barker K.R. (1973). "A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique". Plant Disease Reporter Vol 57, 1025-1028.
22. IBGE (2014). Anuário estatístico do Brasil, ano de 2014. Rio de Janeiro, Brazil, IBGE.
23. Jones J.T., Haegeman A., Danchin E.G., Gauer H.S., Helder J., Jones M.G., Kikuchi T., Manzanilla-López R., Palomares-Rius J.E., Wesemael W.M. , et al (2013). "Top 10 plant-parasitic nematodes in molecular plant pathology". Molecular Plant Pathology Vol 14, 946-961. [Crossref]
24. Koenning S.R., Barker K.R. (1992). "Relative damage functions and reproductive potentials of Meloidogyne arenaria and M. hapla on peanut". Journal of Nematology Vol 24, 187-192.
25. McClure M., Kruk T.H., Migashi I. (1973). "A method for obtaining quantities of clear Meloidogyne eggs". Journal of Nematology Vol 5, 230.
26. Medina I.L., Coila V.H.C., Gomes C.B., Pereira A.S., Nazareno N.R.X. (2014). "Ocorrência de Meloidogyne ethiopica no Paraná e reação de cultivares de batata ao nematoide". Horticultura Brasileira Vol 32, 482-485. [Crossref]
27. Moens M., Perry R.N., Starr J.L. (2009). "Meloidogyne species – a diverse group of novel and important plant parasites". In: Perry R.N., Moens M., Starr J.L. (Eds). Root-knot nematodes. Wallingford, UK, CAB International, pp.  1-17. [Crossref]
28. Molendijk L.P.G., Mulder A. (1996). "The Netherlands, nematodes and potatoes: old problems are here again". Potato Research Vol 39, 471-477. [Crossref]
29. Onkendi E.M., Moleleki L.N. (2013). "Distribution and genetic diversity of root-knot nematodes (Meloidogyne spp.) in potatoes in South Africa". Plant Pathology Vol 62, 1184-1192. [Crossref]
30. Randig O., Bongiovanni M., Carneiro R.M.D.G., Castagnone-Sereno P. (2002). "Genetic diversity of root-knot nematodes from Brazil and development of SCAR markers specific for the coffee-damaging species". Genome Vol 45, 862-870. [Crossref]
31. Saitou N., Nei M. (1987). "The neighbor-joining method, a new method for reconstructing phylogenetic trees". Molecular Biology and Evolution Vol 4, 406-425.
32. Silva A.R., Santos J.M., Hayashi P.C.R., Hayashi E. (2010). "Reação de clones e cultivares de batata avaliados em casa de vegetação a Meloidogyne incognita, M. javanica e M. mayaguensis e in vitro a M. javanica". Nematologia Brasileira Vol 34, 48-55.
33. Silva E.H., Mattos V.S., Furlaneto C., Giband M., Barroso P.A.V., Moita A.W.M., Jorge-Junior A., Correa V.R., Castagnone-Sereno P., Carneiro R.M.D.G. (2014). "Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes". European Journal of Plant Pathology Vol 139, 195-204. [Crossref]
34. Swofford D.L. (2002). PAUP∗: phylogenetic analysis using parsimony (∗and other methods), version 4b10. Sunderland, MA, USA, Sinauer Associates.
35. Triantaphyllou A.C. (1985). "Cytogenetics, cytotaxonomy and phylogeny of root-knot nematodes". In: Sasser J.N., Carter C.C. (Eds). An advanced treatise on Meloidogyne , Vol Vol. 1. Raleigh, NC, USA, North Carolina State University Graphics, pp.  113-126.
36. Viaene N.V., Mahieu T.M., de la Peña E. (2007). "Distribution of Meloidogyne chitwoodi in potato tubers and comparison of extraction methods". Nematology Vol 9, 143-150. [Crossref]
37. Vovlas N., Mifsud D., Landa B.B., Castillo P. (2005). "Pathogenicity of the root-knot nematode Meloidogyne javanica on potato". Plant Pathology Vol 54, 657-664. [Crossref]
38. Wesemael W.M.L., Viaene N., Moens M. (2011). "Root knot nematodes (Meloidogyne spp.) in Europe". Nematology Vol 13, 3-16. [Crossref]
39. Whitehead A.G. (1969). "The distribution of root-knot nematodes (Meloidogyne spp.) in tropical Africa (1969)". Nematologica Vol 15, 315-333. [Crossref]
40. Zijlstra C., Donkers-Venne D.T.H.M., Fargette M. (2000). "Identification of Meloidogyne incognita, M. javanica and M. arenaria using sequence characterised amplified regions (SCAR) based PCR assays". Nematology Vol 2, 847-853. [Crossref]
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2017-01-01
2018-06-22

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