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Molecular and morphological characterisation of Ditylenchus persicus n. sp. (Nematoda: Anguinidae) from Kermanshah province, western Iran

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A new species of the genus Ditylenchus, D. persicus n. sp., was collected around the roots of grapevine and is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 783 (635-928) μm and 689 (670-715) μm in female and male, respectively, delicate stylet 6.2 (5.0-7.0) μm long, six lines in the lateral field, median bulb of pharynx well developed, muscular with crescentic valve, basal pharyngeal bulb pyriform with 10 (8-13) μm long stem extending into intestine, post-vulval uterine sac relatively short, 16 (14-18) μm long, female and male tail elongate-conoid with finely rounded terminus, and bursa covering 65-80% of male tail length. Morphologically, D. persicus n. sp. appears closer to five known species of the genus, namely: D. arachis, D. caudatus, D. clarus, D. myceliophagus and D. nanus. The results of phylogenetic analyses based on sequences of D2-D3 expansion region of 28S rRNA, ITS and partial 18S rRNA genes confirmed the close molecular relationship between D. persicus n. sp. and other Ditylenchus species such as D. myceliophagus, D. africanus, D. arachis, D. destructor and D. halictus.

Affiliations: 1: 1Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran ; 2: 2Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC) Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain

*Corresponding author, e-mail:

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1. Boutsika K., Brown D.J.F., Phillips M.S., Blok V.C. (2004). "Molecular characterisation of the ribosomal DNA of Paratrichodorus macrostylus, P. pachydermus, Trichodorus primitivus and T. similis (Nematoda: Trichodoridae)". Nematology Vol 6, 641-654. [Crossref]
2. Brzeski M.W. (1991). "Review of the genus Ditylenchus Filipjev, 1936 (Nematoda: Anguinidae)". Revue de Nématologie Vol 14, 9-59.
3. Bütschli O. (1873). "Beitrage zur Kenntnis der freilebende nematoden". Nova Acta Academiae Naturale Carolinge Vol 36, 1-124.
4. Castresana J. (2000). "Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis". Molecular Biology and Evolution Vol 17, 540-552. [Crossref]
5. Chizhov V.N., Borisov B.A., Subbotin S.A. (2010). "A new stem nematode, Ditylenchus weischeri sp. n. (Nematoda: Tylenchida), a parasite of Cirsium arvense (L.) Scop in the Central Region of the non-Chernozem zone of Russia". Russian Journal of Nematology Vol 18, 95-102.
6. Darriba D., Taboada G.L., Doallo R., Posada D. (2012). "jModelTest 2: more models, new heuristics and parallel computing". Nature Methods Vol 9, 772. [Crossref]
7. De Grisse A.T. (1969). "Redescription ou modifications de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires". Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit Gent Vol 34, 351-369.
8. De Ley P., Félix M.A., Frisse L.M., Nadler S.A., Sternberg P.W., Thomas K.W. (1999). "Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae)". Nematology Vol 1, 591-612. [Crossref]
9. De Luca F., Reyes A., Troccoli A., Castillo P. (2011). "Molecular variability and phylogenetic relationships among different species and populations of Pratylenchus (Nematoda: Pratylenchidae) as inferred from the analysis of the ITS rDNA". European Journal of Plant Pathology Vol 130, 415-426. [Crossref]
10. Filipjev I.N. (1936). "On the classification of the Tylenchinae". Proceedings of the Helminthological Society of Washington Vol 3, 80-82.
11. Giblin-Davis R.M., Erteld C., Kanzaki N., Ye W., Zeng Y., Center B.J. (2010). "Ditylenchus halictus n. sp. (Nematoda: Anguinidae), an associate of the sweat bee, Halictus sexcinctus (Halictidae), from Germany". Nematology Vol 12, 891-904. [Crossref]
12. Goodey J.B. (1958). "Ditylenchus myceliophagus n. sp. (Nematoda: Tylenchidae)". Nematologica Vol 3, 91-96. [Crossref]
13. Goodey T. (1951). Soil and freshwater nematodes. London, UK, Methuen & Co.
14. Hall T.A. (1999). "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT". Nucleic Acids Symposium Series Vol 41, 95-98.
15. Hooper D.J. (1973). Ditylenchus destructor. CIH Descriptions of plant-parasitic nematodes, Set 2, No. 21. Commonwealth Agricultural Bureaux, Farnham Royal, UK.
16. Katoh K., Standley D.M. (2013). "MAFFT multiple sequence alignment 542 software version 7: improvements in performance and usability". Molecular Biology and Evolution Vol 30, 772-780. [Crossref]
17. Kühn J. (1857). "Über das Vorkommenvon Anguillulen in erkrankten Bluhtenkopfen von Dipsacus fullonum L." Zeitschrift für Wissenschaftliche Zoologie Vol 9, 129-137.
18. Oliveira R.D.L., Santin A.M., Seni D.J., Dietrich A., Salazar L.A., Subbotin S.A., Mundo-Ocampo M., Goldenberg R., Barreto R.W. (2013). "Ditylenchus gallaeformans sp. n. (Tylenchida: Anguinidae) – a neotropical nematode with biocontrol potential against weedy Melastomataceae". Nematology Vol 15, 179-196. [Crossref]
19. Page R.D.M. (1996). "TREEVIEW: an application to display phylogenetic trees on personal computers". Computer Applications in the Biosciences Vol 12, 357-358.
20. Palomares-Rius J.E., Cantalapiedra-Navarrete C., Castillo P. (2014). "Cryptic species in plant-parasitic nematodes". Nematology Vol 16, 1105-1118. [Crossref]
21. Qiao Y., Yu Q., Badiss A., Zaidi M.A., Ponomareva E., Hu Y., Ye W. (2016). "Paraphyletic genus Ditylenchus Filipjev (Nematoda, Tylenchida), corresponding to the D. triformis-group and the D. dipsaci-group scheme". ZooKeys Vol 568, 1-12. [Crossref]
22. Ronquist F., Huelsenbeck J.P. (2003). "MrBAYES3: Bayesian phylogenetic inference under mixed models". Bioinformatics Vol 19, 1572-1574. [Crossref]
23. Siddiqi M.R. (1963). "Four new species in the subfamily Tylenchinae (Nematoda) from north India". Zeitschrift für Parasitenkunde Vol 23, 397-404. [Crossref]
24. Siddiqi M.R. (2000). Tylenchida parasites of plants and insects, 2nd edition. Wallingford, UK, CABI Publishing. [Crossref]
25. Sturhan D., Brzeski M.W. (1991). "Stem and bulb nematodes, Ditylenchus spp." In: Nickle W.R. (Ed.). Manual of agricultural nematology. New York, NY, USA, Marcel Dekker, pp.  423-464.
26. Subbotin S.A., Krall E.L., Riley I.T., Chizhov V.N., Staelens A., De Loose M., Moens M. (2004). "Evolution of the gall-forming plant parasitic nematodes (Tylenchida: Anguinidae) and their relationships with hosts as inferred from Internal Transcribed Spacer sequences of nuclear ribosomal DNA". Molecular Phylogenetics and Evolution Vol 30, 226-235. [Crossref]
27. Subbotin S.A., Madani M., Krall E., Sturhan D., Moens M. (2005). "Molecular diagnostics, taxonomy and phylogeny of the stem nematode Ditylenchus dipsaci species complex based on the sequences of the internal transcribed spacer-rDNA". Phytopathology Vol 95, 1308-1315. [Crossref]
28. Subbotin S.A., Sturhan D., Chizhov V., Vovlas N., Baldwin J. (2006). "Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences". Nematology Vol 8, 455-474. [Crossref]
29. Thorne G. (1934). "Some plant-parasitic nemas, with descriptions of three new species". Journal of Agricultural Research Vol 49, 755-763.
30. Thorne G. (1945). "Ditylenchus destructor, n. sp. the potato rot nematode, and Ditylenchus dipsaci (Kühn, 1957) Filipjev, 1936, the teasel nematode (Nematoda: Tylenchidae)". Proceedings of the Helminthological Society of Washington Vol 12, 27-34.
31. Thorne G., Malek R.B. (1968). Nematodes of the Northern Great Plains. Part I. Tylenchida [Nemata: Secernentea]. Agricultural Experimental Station Bulletin 31, South Dakota State Unniversity, Brookings, SD, USA.
32. Tzortzakakis E.A., Archidona-Yuste A., Liébanas G., Birmpilis I.G., Cantalapiedra-Navarrete C., Navas-Cortés J.A., Castillo P., Palomares-Rius J.E. (2016). "Rotylenchus cretensis n. sp. and R. cypriensis Antoniou 1980 (Nematoda: Hoplolaimidae) recovered from the rhizosphere of olive at Crete (Greece) with a molecular phylogeny of the genus". European Journal of Plant Pathology Vol 144, 167-184. [Crossref]
33. Vovlas N., Troccoli A., Palomares-Rius J.E., De Luca F., Liébanas G., Landa B.B., Subbotin S.A., Castillo P. (2011). "Ditylenchus gigas n. sp. parasitizing broad bean: a new stem nematode singled out from the Ditylenchus dipsaci species complex using a polyphasic approach with molecular phylogeny". Plant Pathology Vol 60, 762-775. [Crossref]
34. Vovlas N., Troccoli A., Palomares-Rius J.E., De Luca F., Cantalapiedra-Navarrete C., Liébanas G., Landa B.B., Subbotin S.A., Castillo P. (2016). "A new stem nematode, Ditylenchus oncogenus n. sp. (Nematoda: Tylenchida), parasitizing sowthistle from Adriatic coast dunes in southern Italy". Journal of Helminthology Vol 90, 152-165. [Crossref]
35. Wendt K.R., Swart A., Vrain T.C., Webster J.M. (1995). "Ditylenchus africanus sp. n. from South Africa; a morphological and molecular characterization". Fundamental and Applied Nematology Vol 18, 241-250.
36. Whitehead A.G., Hemming J.R. (1965). "A comparison of some quantitative methods of extracting small vermiform nematodes from soil". Annals of Applied Biology Vol 55, 25-38. [Crossref]
37. Zhang S.L., Liu G.K., Janssen T., Zhang S.S., Xiao S., Li S.T., Couvreur M., Bert W. (2014). "A new stem nematode associated with peanut pod rot in China: morphological and molecular characterization of Ditylenchus arachis n. sp. (Nematoda: Anguinidae)". Plant Pathology Vol 63, 1193-1206. [Crossref]

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