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Morphological and molecular characterisation of Heterodera koreana (Vovlas, Lamberti & Choo, 1992) Mundo-Ocampo, Troccoli, Subbotin, Del Cid, Baldwin & Inserra, 2008 (Nematoda: Heteroderidae) from bamboo in Japan

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The Korean cyst nematode, Heterodera koreana, was recorded for the first time from Japan and characterised morphologically, morphometrically and molecularly. In total, 41 populations were detected from soil samples collected from the rhizosphere of four bamboo species in Japan: 31 populations from moso bamboo (Phyllostachys edulis), seven from madake (P. bambusoides), two from henon bamboo (P. nigra var. henonis) and one from fish pole bamboo (P. aurea). The morphology and morphometrics of the Japanese population were in agreement with those of the original description of H. koreana from South Korea and other subsequent descriptions from China and Iran, with the exception of some minor differences. The results of the phylogenetic analyses of the D2-D3 expansion segments of 28S rRNA gene and ITS rRNA gene sequences confirmed the species identification and phylogenetic relationship of H. koreana with other Heterodera species. The COI mtDNA gene sequences were obtained for the first time for H. koreana. Three COI haplotypes found in Japanese H. koreana populations showed a characteristic geographical distribution in Japan.

Affiliations: 1: 1Central Region Agricultural Research Center (CARC), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8666, Japan ; 2: 2Maruwa Biochemical Co., Ltd., Ami Development Center, 1 Hoshinosato, Ami, Ibaraki 305-0326, Japan

*Corresponding author, e-mail:

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1. Aiba S. (2014). ["Extraction of cyst nematodes".] In: Mizukubo T., Futai K. (Eds). [ Nematological experimentation.] Kyoto, Japan, Kyoto University Press, pp.  199-200.
2. Armenteros M., Rojsa-Corzo A., Ruiz-Abierno A., Derycke S., Backeljau T., Decraemer W. (2014). "Systematics and DNA barcoding of free-living marine nematodes with emphasis on tropical desmodorids using nuclear SSU rDNA and mitochondrial COI sequences". Nematology Vol 16, 979-989. DOI: [Crossref]
3. De Ley P., Félix M.A., Frisse L.M., Nadler S.A., Sternberg P.W., Thomas W.K. (1999). "Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae)". Nematology Vol 1, 591-612. DOI: [Crossref]
4. De Luca F., Vovlas N., Lucarelli G., Troccoli A., Radicci V., Fanelli E., Cantalapiedra-Navarrete C., Palomares-Rius J.E., Castillo P. (2013). "Heterodera elachista the Japanese cyst nematode parasitizing corn in Northern Italy: integrative diagnosis and bionomics". European Journal of Plant Pathology Vol 136, 857-872. DOI: [Crossref]
5. Derycke S., Remerie T., Vierstraete A., Backeljau T., Vanfleteren J., Vincx M., Moens T. (2005). "Mitochondrial DNA variation and cryptic speciation within the free-living marine nematode Pellioditis marina". Marine Ecology Progress Series Vol 300, 91-103. [Crossref]
6. Edgar R.C. (2004). "MUSCLE: multiple sequence alignment with high accuracy and high throughput". Nucleic Acids Research Vol 32, 1792-1797. DOI: [Crossref]
7. Inserra R.N., Vovlas N., Lehman P.S. (1999). "Parasitism of fishpole bamboo roots by Afenestrata koreana". Nematropica Vol 29, 105-111.
8. Iwahori H., Kanzaki N., Futai K. (2000). "A simple, polymerase chain reaction-restriction fragment length polymorphism-aided diagnosis method for pine wilt disease". Forest Pathology Vol 30, 157-164. DOI: [Crossref]
9. Joyce S.A., Reid A., Driver F., Curran J. (1994). "Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes". In: Burnell A.M., Ehlers R.-U., Masson J.P. (Eds). Cost 812 biotechnology: genetics of entomopathogenic nematode-bacteria complexes. Proceedings of Symposium & Workshop, St. Patrick’s College, Maynooth, CO. Kildare, Ireland. Luxembourg, European Commission, pp.  178-187.
10. Kaushall K.K., Swarup G. (1988). "Two new cyst nematode species from India". Indian Journal of Nematology Vol 18, 299-306.
11. Madani M., Vovlas N., Castillo P., Subbotin S.A., Moens M. (2004). "Molecular characterization of cyst nematode species (Heterodera spp.) from the Mediterranean basin using RFLPs and sequences of ITS-rDNA". Journal of Phytopathology Vol 152, 229-234. DOI: [Crossref]
12. Minagawa N., Mizukubo T. (1994). "A simplified procedure of transferring nematodes to glycerol for permanent mounts". Japanese Journal of Nematology Vol 24, 75. [Crossref]
13. Momota Y. (2004). "Identification of cyst nematodes in Japan". In: Japan Plant Protection Association (Ed.). [ Identification of nematode species (Shokubutsu Boeki (Plant Protection) special extra edition No. 8).] Tokyo, Japan, Japan Plant Protection Association, pp.  23-28.
14. Mundo-Ocampo M., Troccoli A., Subbotin S.A., Del Cid J., Baldwin J.G., Inserra R.N. (2008). "Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb. n. and H. orientalis comb. n. (Tylenchida: Heteroderidae)". Nematology Vol 10, 611-632. DOI: [Crossref]
15. Nylander J.A.A. (2004). MrModeltest v2. Program distributed by the author. Uppsala, Sweden, Evolutionary Biology Centre, Uppsala University.
16. Orlando V., Chitambar J.J., Dong K., Chizhov V.N., Mollov D., Bert W., Subbotin S.A. (2016). "Molecular and morphological characterisation of Xiphinema americanum-group species (Nematoda: Dorylaimida) from California, USA, and other regions, and co-evolution of bacteria from the genus Candidatus Xiphinematobacter with nematodes". Nematology Vol 18, 1015-1043. DOI: [Crossref]
17. Rambaut A. (2009). FigTree v1.3.1. Available online at
18. Rambaut A., Drummond A. (2013). Tracer v1.6. Available online at
19. Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A., Huelsenbeck J.P. (2011). "MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space". Systematic Biology Vol 61, 539-542. DOI: [Crossref]
20. Sakai H. (2010). "A DNA extraction method with SDS from single nematodes for direct application to PCR amplification". Nematological Research Vol 40, 13-14. DOI: [Crossref]
21. Sakai H., Takeda A., Mizukubo T. (2012). "Intra-specific variation of Xiphinema brevicolle Lordello et Costa, 1961 (Nematoda: Longidoridae) in Japan". Nematological Research Vol 42, 1-7. DOI: [Crossref]
22. Sekimoto S., Uehara T., Mizukubo T. (2016). "Geographical distribution of Heterodera trifolii in eastern Japan". Nematological Research Vol 46, 1-8. [Crossref]
23. Siddiqi M.R. (2000). Tylenchida parasites of plants and insects, 2nd edition. Wallingford, UK, CABI Publishing. [Crossref]
24. Skantar A.M., Handoo Z.A., Zanakis G.N., Tzortzakakis E.A. (2012). "Molecular and morphological characterization of the corn cyst nematode, Heterodera zeae, from Greece". Journal of Nematology Vol 44, 58-66.
25. Sturhan D. (2010). "Notes on morphological characteristics of 25 cyst nematodes and related Heteroderidae". Russian Journal of Nematology Vol 18, 1-8.
26. Subbotin S.A. (2015). "Heterodera sturhani sp. n. from China, a new species of the Heterodera avenae species complex (Tylenchida: Heteroderidae)". Russian Journal of Nematology Vol 23, 145-152.
27. Subbotin S.A., Vierstraete A., De Ley P., Rowe J., Waeyenberge L., Moens M., Vanfleteren J.R. (2001). "Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA". Molecular Phylogenetics and Evolution Vol 21, 1-16. DOI: [Crossref]
28. Subbotin S.A., Sturhan D., Rumpenhorst H.J., Moens M. (2003). "Molecular and morphological characterisation of the Heterodera avenae species complex (Tylenchida: Heteroderidae)". Nematology Vol 5, 515-538. DOI: [Crossref]
29. Subbotin S.A., Sturhan D., Chizhov V.N., Vovlas N., Baldwin J.G. (2006). "Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences". Nematology Vol 8, 455-474. DOI: [Crossref]
30. Subbotin S.A., Mundo-Ocampo M., Baldwin J.G. (2010). Systematics of cyst nematodes (Nematoda: Heteroderinae). Nematology Monographs and Perspectives 8B (Series Editors: Hunt D.J., Perry R.N.). Leiden, The Netherlands, Brill.
31. Subbotin S.A., Stanley J.D., Ploeg A.T., Tanha Maafi Z., Tzortzakakis E.A., Chitambar J.J., Palomares-Rius J.E., Castillo P., Inserra R.N. (2015). "Characterisation of populations of Longidorus orientalis Loof, 1982 (Nematoda: Dorylaimida) from date palm (Phoenix dactylifera L.) in the USA and other countries and incongruence of phylogenies inferred from ITS1 rRNA and coxI genes". Nematology Vol 17, 459-477. DOI: [Crossref]
32. Swofford D.L. (2003). PAUP∗: phylogenetic analysis using parsimony (∗and other methods), Version 4.0b 10. Sunderland, MA, USA, Sinauer Associates.
33. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011). "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods". Molecular Biology and Evolution Vol 28, 2731-2739. DOI: [Crossref]
34. Tanha Maafi Z., Majd Taheri Z. (2015). "First report of Korean cyst nematode, Heterodera koreana, parasitic on bamboo, Phyllostachys nigra, from Iran". Journal of Nematology Vol 47, 167-168.
35. Tanha Maafi Z., Subbotin S.A., Moens M. (2003). "Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences". Nematology Vol 5, 99-111. DOI: [Crossref]
36. Toumi F., Waeyenberge L., Viaene N., Dababat A., Nicol J.M., Ogbonnaya F., Moens M. (2013). "Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi". European Journal of Plant Pathology Vol 136, 613-624. DOI: [Crossref]
37. Turner S.J., Subbotin S.A. (2013). "Cyst nematodes". In: Perry R.N., Moens M. (Eds). Plant nematology, 2nd edition. Wallingford, UK, CAB International, pp.  109-143. [Crossref]
38. Vovlas N., Lamberti F., Choo H.Y. (1992). "Description of Afenestrata koreana n. sp. (Nematoda: Heteroderinae), parasite of bamboo in Korea". Journal of Nematology Vol 24, 553-559.
39. Vovlas N., Vovlas A., Leonetti P., Liebanas G., Castillo P., Subbotin S.A., Palomares-Rius J.E. (2015). "Parasitism effects on white clover by root-knot and cyst nematodes and molecular separation of Heterodera daverti from H. trifolii". European Journal of Plant Pathology Vol 143, 833-845. DOI: [Crossref]
40. Wang H.H., Zhuo K., Zhang H.L., Liao J.L. (2012). "Heterodera koreana, a new record species from China". Acta Phytopathologica Sinica Vol 42, 551-555.
41. Wang H.H., Zhuo K., Ye W.M., Zhang H.L., Peng D.L., Liao J.L. (2013). "Heterodera fengi n. sp. (Nematoda: Heteroderinae) from bamboo in Guangdong province, China – a new cyst nematode in the Cyperi group". Zootaxa Vol 3652, 179-192. DOI: [Crossref]
42. Zheng J.W., Subbotin S.A., Waeyenberge L., Moens M. (2000). "Molecular characterisation of Chinese Heterodera glycines and H. avenae populations based on RFLPs and sequences of rDNA-ITS regions". Russian Journal of Nematology Vol 8, 109-113.
43. Zheng J.W., Li X.Q., Zhang Y., Subbotin S.A. (2008). "Molecular characterization of the cyst forming nematode, Heterodera sinensis Chen & Zheng, 1994, from China". Russian Journal of Nematology Vol 16, 159-162.
44. Zhuo K., Wang H.H., Ye W., Peng D.L., Liao J.L. (2013). "Heterodera hainanensis n. sp. (Nematoda: Heteroderinae) from bamboo in Hainan province, China – a new cyst nematode in the Afenestrata group". Nematology Vol 15, 303-314. DOI: [Crossref]
45. Zhuo K., Wang H.H., Zhang H.L., Liao J.L. (2014). "Heterodera guangdongensis n. sp. (Nematoda: Heteroderinae) from bamboo in Guangdong province, China – a new cyst nematode in the Cyperi group". Zootaxa Vol 3881, 488-500. DOI: [Crossref]

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