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Comparisons between two Antarctic nematodes: cultured Panagrolaimus sp. DAW1 and field-sourced Panagrolaimus davidi

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The Antarctic nematode Panagrolaimus davidi can survive intracellular freezing. Genetic studies indicate the culture strain (now designated as Panagrolaimus sp. DAW1) is a different species to the P. davidi of field origin. This paper reports further attempts both to isolate DAW1 from Antarctic soils and to culture P. davidi itself. Sequencing of the 18S rRNA gene of 151 individuals indicates that DAW1 is rare in the field, but characterising two new isolates shows that, nevertheless, it is present. Panagrolaimus davidi is common in the field, but cannot be cultured using the media tested here. These two species are difficult to distinguish morphologically, apart from the absence of males in DAW1 and its longer recurved tail. Whilst it is possible that DAW1 is an introduced species, the sites at Shackleton’s hut at Cape Royds are dominated by P. davidi, which is clearly an endemic species.

Affiliations: 1: 1Department of Zoology, Otago, University of Otago, P.O. Box 56, Dunedin, New Zealand ; 2: 2Department of Biochemistry/Genetics, Otago, University of Otago, P.O. Box 56, Dunedin, New Zealand

*Corresponding author, e-mail: david.wharton@otago.ac.nz
**Present address: CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, 4485-661 Vairão, Portugal.
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1. Ali F., Wharton D.A. (2014). "Intracellular freezing in the infective juveniles of Steinernema feltiae: an entomopathogenic nematode". PLoS ONE Vol 9(4), e94179. DOI:
2. Barrière A., Félix M.-A. (2014). Isolation of C. elegans and related nematodes. In: The C. elegans Research Community (Ed.). WormBook, available online at http://www.wormbook.org. DOI:
3. Edgar R.C. (2004). "MUSCLE: multiple sequence alignment with high accuracy and high throughput". Nucleic Acids Research Vol 32, 1792-1797. DOI: [Crossref]
4. Floyd R.M., Rogers A.D., Lambshead P.J.D., Smith C.R. (2005). "Nematode-specific PCR primers for the 18S small subunit rRNA gene". Molecular Ecology Notes Vol 5, 611-612. DOI: [Crossref]
5. Hall T. (1999). "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT". Nucleic Acids Symposium Series Vol 41, 95-98.
6. Hooper D.J. (1986a). "Extraction of free-living stages from soil". In: Southey J.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UK, HMSO, pp.  5-30.
7. Hooper D.J. (1986b). "Handling, fixing, staining and mounting nematodes". In: Southey J.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UK, HMSO, pp.  59-85.
8. Hooper D.J. (1986c). "Drawing and measuring nematodes". In: Southey J.F. (Ed.). Laboratory methods for work with plant and soil nematodes. London, UK, HMSO, pp.  87-94.
9. Lewis S.C., Dyal L.A., Hilburn C.F., Weitz S., Liau W.S., LaMunyon C.W., Denver D.R. (2009). "Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the Antarctic species P. davidi". BMC Evolutionary Biology Vol 9, 15. DOI: [Crossref]
10. McGill L.M., Shannon A.J., Pisani D., Félix M.A., Ramløv H., Dix I., Wharton D.A., Burnell A.M. (2015). "Anhydrobiosis and freezing-tolerance: adaptations that facilitate the establishment of Panagrolaimus nematodes in polar habitats". PLoS ONE Vol 10(3), e0116084. DOI: [Crossref]
11. Piggott S.J., Perry R.N., Wright D.J. (2000). "Hypo-osmotic regulation in entomopathogenic nematodes: Steinernema spp. and Heterorhabditis spp". Nematology Vol 2, 561-566. DOI: [Crossref]
12. Porazinska D.L., Wall D.H., Virginia R.A. (2002). "Invertebrates in ornithogenic soils on Ross Island, Antarctica". Polar Biology Vol 25, 569-574. DOI:
13. Rasband W.S. (1997-2005). ImageJ. Available online at http://rsb.info.nih.gov/ij/.
14. Raymond M.R., Wharton D.A., Marshall C.J. (2013). "Factors determining nematode distributions at Cape Hallett and Gondwana station, Antarctica". Antarctic Science Vol 25, 347-357. DOI: [Crossref]
15. Raymond M.R., Wharton D.A., Marshall C.J. (2014). "Nematodes from the Victoria land coast, Antarctica and comparisons with cultured Panagrolaimus davidi". Antarctic Science Vol 26, 15-22. DOI: [Crossref]
16. Salehian S., Braeckman B.P., Beladjal L., Bert W., Clegg J.S., Mertens J. (2011). "The importance of feeding status and desiccation rate in successful anhydrobiosis of Panagrolaimus detritophagus". Nematology Vol 13, 185-191. DOI: [Crossref]
17. Smith T., Wharton D.A., Marshall C.J. (2008). "Cold tolerance of an Antarctic nematode that survives intracellular freezing: comparisons with other nematode species". Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology Vol 178, 93-100. DOI: [Crossref]
18. Stiernagle T. (1999). "Maintenance of C. elegans". In: Hope I.A. (Ed.). C. elegans: a practical approach. Oxford, UK, Oxford University Press, pp.  51-67.
19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. (2013). "MEGA6: molecular evolutionary genetics analysis version 6.0". Molecular Biology and Evolution Vol 30, 2725-2729. DOI: [Crossref]
20. Timm R.W. (1971). "Antarctic soil and freshwater nematodes from the McMurdo sound region". Proceedings of the Helminthological Society of Washington Vol 38, 42-52.
21. Wharton D.A. (1998). "Comparison of the biology and freezing tolerance of Panagrolaimus davidi, an Antarctic nematode, from field samples and cultures". Nematologica Vol 44, 643-653. DOI: [Crossref]
22. Wharton D.A. (2003). "The environmental physiology of Antarctic terrestrial nematodes: a review". Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology Vol 173, 621-628. DOI: [Crossref]
23. Wharton D.A. (2011). "Cold tolerance". In: Perry R.N., Wharton D.A. (Eds). Molecular and physiological basis of nematode survival. Wallingford, UK, CABI Publishing, pp.  182-204. [Crossref]
24. Wharton D.A., Brown I.M. (1989). "A survey of terrestrial nematodes from the McMurdo sound region, Antarctica". New Zealand Journal of Zoology Vol 16, 467-470. [Crossref]
25. Wharton D.A., Ferns D.J. (1995). "Survival of intracellular freezing by the Antarctic nematode Panagrolaimus davidi". Journal of Experimental Biology Vol 198, 1381-1387.
26. Wharton D.A., Raymond M.R. (2015). "Cold tolerance of the Antarctic nematodes Plectus murrayi and Scottnema lindsayae". Journal of Comparative Physiology B–Biochemical Systemic and Environmental Physiology Vol 185, 281-289. DOI: [Crossref]
27. Yoder M., De Ley I.T., King I.W., Mundo-Ocampo M., Mann J., Blaxter M., Poiras L., De Ley P. (2006). "DESS: a versatile solution for preserving morphology and extractable DNA of nematodes". Nematology Vol 8, 367-376. DOI: [Crossref]
28. Zhang Z., Schwartz S., Wagner L., Miller W. (2000). "A greedy algorithm for aligning DNA sequences". Journal of Computational Biology Vol 7, 203-214. DOI: [Crossref]
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2017-06-06
2017-11-18

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