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Morphological and molecular characterisation of Longidorus juglans sp. nov. and a sister species L. fangi Xu & Cheng, 1991 (Nematoda: Longidoridae) from China

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Longidorus juglans sp. nov. is a new needle nematode detected from the rhizosphere of a walnut tree in Shanxi Province, P.R. China. It was distinguished by female body length of 3.9-5.2 mm; lip region 14-18 μm wide, rounded and continuous, amphidial fovea pocket-shaped without distinct basal lobes; moderately long odontostyle (125-140 μm); guide ring at mid-odontostyle and situated 69-78 μm from anterior end; short and blunt conoid tail (30-41 μm long, c = 118-147, c′ = 0.6-0.9), bearing 2-3 caudal pores on each side. The male of L. juglans sp. nov. is 5.1 mm long, with 13 ventro-median supplements, a short and blunt conoid tail bearing 3-4 caudal pores on each side, and spicules 73 μm long. Three developmental juvenile stages were recognised. This new species is very similar to another Chinese species L. fangi in general morphology, but can be distinguished by the number of juvenile stages and DNA sequences of SSU, ITS1, ITS2 and D2-D3 of LSU rDNA. Morphological and molecular characterisation was provided for L. fangi representing two new geographical records. The phylogenetic relationships among these species were analysed using data from SSU, D2-D3 of LSU, ITS1 and ITS2 rDNA, which revealed that L. juglans sp. nov. and L. fangi were clustered together with L. diadecturus, L. litchii and L. jonesi whose guide ring is located at mid-odontostyle level.

Affiliations: 1: 1Laboratory of Nematology, Department of Plant Pathology, Agronomy College, Shanxi Agricultural University, Taigu, 030801, P.R. China ; 2: 2State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin’an, Hangzhou 311300, P.R. China ; 3: 3Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P.R. China ; 4: 4Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC 27607, USA ; 5: 5Landcare Research, Private Bag 92170, Auckland Mail Centre, Auckland 1142, New Zealand

*Corresponding authors, e-mails:;

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