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Reproductive, pathogenic and genotypic characterisation of five Meloidogyne graminicola populations from the Philippines on susceptible and resistant rice varieties

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Five populations of Meloidogyne graminicola isolated from different rice-growing areas in the Philippines were characterised. The populations showed little phenotypic variability of second-stage juveniles and female perineal pattern. Differences in reproduction among M. graminicola populations were not observed on mature resistant Oryza glaberrima varieties ‘TOG5674’, ‘TOG5675’, ‘RAM131’ and ‘CG14’, or on susceptible O. sativa varieties ‘IR64’ and ‘UPLRi-5’. In all infected rice varieties, plant growth and yield-contributing traits showed no differences among the populations. A search on M. graminicola populations from the Philippines for single-nucleotide polymorphism on the sequences of Internal Transcribed Spacer (ITS) of rDNA genes and mtDNA indicated only few points of heteroplasmy. Nematode reproduction and disease induction of the five M. graminicola populations in the Philippines exerted the same level of aggressiveness and virulence. The absence of resistance-breaking populations of M. graminicola is important for the maintenance of durability of resistance to this important rice pathogen.

Affiliations: 1: 1Laboratory of Tropical Crop Improvement, Department of Biosystems, Faculty of Bioscience Engineering, University of Leuven (KU Leuven), Willem de Croylaan 42, 3001 Heverlee, Belgium ; 2: 2International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines ; 3: 3Department of Biological Sciences, College of Arts and Sciences, University of Southern Mindanao, Kabacan 9407, Cotabato, Philippines ; 4: 4Interactions Plantes-Microorganismes-Environnement, Institut de Recherche pour le Développement (IRD), Cirad, Univ Montpellier, Montpellier, France ; 5: 5Laboratoire Mixte International RICE2, Agriculture Genetics Institute (AGI), Hanoi, Vietnam ; 6: 6Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, 2520 Potchefstroom, South Africa

*Corresponding author, e-mail:

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