Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Morphological and molecular characterisation of two new Hemicycliophora species (Tylenchida: Hemicycliophoridae) with a revision of the taxonomic status of some known species and a phylogeny of the genus

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

For more content, see Nematologica.

Hemicycliophora presently contains 132 valid species of sheath nematodes. Within several samples obtained from surveys in Canada, South Africa, Spain and the USA, we distinguished six valid and six putative unidentified species by integrating the results of morphological and molecular analyses. Valid species included: H. californica, H. gracilis, H. parvana, H. poranga, H. raskii, and H. signata. The putative unidentified species were indicated as Hemicycliophora sp. 10, sp. 12, sp. 15, sp. 16, sp. 17, and sp. 18. Two new species of sheath nematodes from Spain and the USA were described and named as H. onubensis sp. n. and H. robbinsi sp. n., respectively. Hemicycliophora wyei is proposed as a junior synonym of H. parvana and H. ripa is proposed as a junior synonym of H. poranga. Eighteen valid and 13 unidentified species of sheath nematodes were characterised using the partial COI mtDNA gene. A total of 94 new sequences of which 77 were for the COI mtDNA gene were obtained in this study. Phylogenetic relationships within Hemicycliophora, using the D2-D3 expansion segments of 28S rDNA, ITS rRNA and COI gene sequences, are presented as inferred from Bayesian analysis.

Affiliations: 1: 1National Collection of Nematodes, Biosystematics Programme, ARC-Plant Protection Research Institute, Private Bag X 134, Queenswood 0121, Pretoria, South Africa ; 2: 2Laboratory for Electron Microscopy, North West University, Potchefstroom Campus, Potchefstroom 2520, South Africa ; 3: 3Department of Animal Biology, Vegetal Biology and Ecology, University of Jaén, Campus ‘Las Lagunillas’ s/n, Edificio B3, 23071 Jaén, Spain ; 4: 4Plant Pest Diagnostic Center, California Department of Food and Agriculture, CA 95832-1448, USA ; 5: 5Florida Department of Agriculture and Consumer Services, DPI, Nematology Section, P.O. Box 147100, Gainesville, FL 32614-7100, USA ; 6: 6Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Campus de Excelencia Internacional Agroalimentario, ceiA3, Avenida Menéndez Pidal s/n, 14004 Córdoba, Spain ; 7: 7Center of Parasitology, A.N Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii Prospect 33, Moscow, 117071, Russia

*Corresponding author, e-mail:

Full text loading...


Data & Media loading...

1. Abolafia J., Liébanas G., Peña-Santiago R. (2002). "Nematodes of the order Rhabditida from Andalucía Oriental, Spain. The subgenus Pseudacrobeles Steiner, 1938, with description of a new species". Journal of Nematode Morphology and Systematics Vol 4, 137-154.
2. Bajaj H.K. (1998). "Criconematids of Kalesar forests and Morni hills of Haryana, India". Indian Journal of Nematology Vol 28, 150-158.
3. Brzeski M.W. (1974). "Taxonomy of the Hemicycliophorinae (Nematoda, Tylenchida)". Problemowe Postepow Nauk Rolniczych Vol 154, 237-330.
4. Castillo P., Gomez-Barcina A., Loof P.A.A. (1989). "On two species of Hemicycliophora de Man, 1921 (Nematoda: Criconematoidea) found in Spain". Nematologia Mediterranea Vol 17, 77-82.
5. Castillo P., Vovlas N., Subbotin S., Troccoli A. (2003). "A new root-knot nematode, Meloidogyne baetica n. sp. (Nematoda: Heteroderidae), parasitizing wild olive in southern Spain". Phytopathology Vol 93, 1093-1102. DOI: 10.1094/PHYTO.2003.93.9.1093 [Crossref]
6. Chaves E. (1983). "Criconematoidea (Nematoda) from Argentina". Nematologica Vol 29, 404-424. DOI: 10.1163/187529283X00285 [Crossref]
7. Chitambar J.J., Subbotin S.A. (2014). Systematics of the sheath nematodes of the superfamily Hemicycliophoroidea. Nematology Monographs and Perspectives 10 (Series Editors: Hunt D.J., Perry R.N.). Leiden, The Netherlands, Brill.
8. Colbran R.C. (1960). "Studies of plant and soil nematodes. 3. Belonolaimus hastulatus, Psilenchus tumidus and Hemicycliophora labiata, three new species from Queensland". Queensland Journal of Agricultural Science Vol 17, 175-181.
9. Coolen W.A. (1979). "Methods for extraction of Meloidogyne spp. and other nematodes from roots and soil". In: Lamberti F., Taylor C.E. (Eds). Root-knot nematodes (Meloidogyne species). Systematics, biology, and control. London, UK, Academic Press, pp.  317-329.
10. Cordero López M.A., Robbins R.T., Szalanski A.L. (2013). "Taxonomic and molecular identification of Hemicycliophora, Caloosia, Gracilacus and Paratylenchus species (Nematoda: Criconematidae)". Journal of Nematology Vol 45, 145-171.
11. Curran J., Driver F., Ballard J.W.O., Milner R.J. (1994). "Phylogeny of Metarhizium: analysis of ribosomal DNA sequence data". Mycological Research Vol 98, 547-555. DOI: 10.1016/S0953-7562(09)80478-4 [Crossref]
12. De Grisse A.T. (1969). "Redescription ou modification de quelques techniques utilisées dans l’étude des nématodes phytoparasitaires". Mededelingen van de Rijksfaculteit der Landbouwwetenschappen Gent Vol 34, 351-369.
13. de Man J.G. (1921). "Nouvelles recherches sur les nématodes libres terricoles de la Hollande". Capita Zoologica Vol 1, 3-62.
14. Doucet M.E. (1982). "Quatre nouvelles espèces du genre Hemicycliophora de Man, 1921 (Nematoda: Tylenchida) provenant d’Argentine". Revue de Nématologie Vol 5, 309-320.
15. Goffart H. (1951). "Nematoden aus unterirdischen Gewassern". Deutsche Zoologische Zeitschrift Vol 1, 73-78.
16. Goodey J.B. (1963). Soil and freshwater nematodes, 2nd edition. London, UK, Methuen.
17. Heyns J. (1962). "Two new species of Criconematidae from South Africa". Nematologica Vol 8, 21-24. DOI: 10.1163/187529262X00963 [Crossref]
18. Jairajpuri M.S., Baqri Q.H. (1973). "Nematodes of high altitudes in India. I. Four new species of Tylenchida". Nematologica Vol 19, 19-30. DOI: 10.1163/187529273X00051 [Crossref]
19. Kirjanova E.S., Shagalina L.M. (1974). ["Hemicycliophora pruni sp. nov. (Nematoda: Hemicycliophoridae) – a parasite of plum tree in central Kopetdagh".] Izvestiya Akademii Nauk Turkmenskoi SSR, Serija Biologicheskikh Nauk Vol 6, 35-39.
20. Loof P.A.A. (1968). Taxonomy of Hemicycliophora species from west and central Europe (Nematoda: Criconematoidea). Mededelingen Landbouwhogeschool Wageningen 68-14, 52 pp.
21. Loof P.A.A. (1984). "Hemicycliophora species from Iran (Nematoda: Criconematoidea)". Nematologica Vol 30, 22-41. DOI: 10.1163/187529284X00437 [Crossref]
22. Loof P.A.A., De Grisse A. (1989). "Taxonomic and nomenclatorial observations on the genus Criconemella De Grisse & Loof, 1965, sensu Luc & Raski, 1981". Mededelingen Faculteit Landbouwwetenschappen Rijksuniversiteit, Gent Vol 54, 53-74.
23. Loof P.A.A., Heyns J. (1969). "Taxonomy of Hemicycliophora species from South Africa (Nematoda: Criconematoidea)". Nematologica Vol 15, 464-472. DOI: 10.1163/187529269X00795 [Crossref]
24. Loos C.A. (1948). "Notes on free-living and plant-parasitic nematodes of Ceylon 3". Ceylon Journal of Science Vol 23, 119-124.
25. Ma X., Agudelo P. (2015). "First report of Hemicycliophora wyei on bentgrass in Texas". Plant Disease Vol 99, 732. DOI: 10.1094/PDIS-09-14-0972-PDN [Crossref]
26. Mehta U.K., Raski D.J., Valenzuela A.A. (1983). "Five new species of Criconematidae (Nemata) from southern Chile". Nematologica Vol 28(1982), 398-411. DOI: 10.1163/187529282X00204
27. Micoletzky H. (1913). "Die freilebenden Süsswassernematoden der Ost-Alpen. 1. Teil der vorlaüfi ge Mitteilung: Die frilebenden Süsswassernematoden des Lunzer Seengebietes". Sitzungsberichte der K. Akademie der Wissenschaften in Wien, Mathematisch-Naturwissenschaftliche-Klasse, Abt. 1 Vol 122(1), 111-122; 122(4), 543-548.
28. Monteiro A.R., Lordello L.G.E. (1978). "A description of Hemicycliophora poranga n. sp. from Brazil (Nemata)". Revista Brasileira de Biologia Vol 38, 569-571.
29. Netscher C., Seinhorst J.W. (1969). "Propionic acid better than acetic acid for killing nematodes". Nematologica Vol 15, 286.
30. Orton Williams K.J. (1978). "Two new species of the genus Hemicycliophora de Man, 1921 (Nematoda: Tylenchida)". Revue de Nématologie Vol 1, 197-205.
31. Powers T.O., Bernard E.C., Harris T., Higgins R., Olson M., Lodema M., Mullin P., Sutton L., Powers K.S. (2014). "COI haplotype groups in Mesocriconema (Nematoda: Criconematidae) and their morphospecies associations". Zootaxa Vol 3827, 101-146. DOI: 10.11646/zootaxa.3827.2.1 [Crossref]
32. Powers T.O., Bernard E.C., Harris T., Higgins R., Olson M., Olson S., Lodema M., Matczyszyn J., Mullin P., Sutton L. , et al  (2016a). "Species discovery and diversity in Lobocriconema (Criconematidae: Nematoda) and related plant-parasitic nematodes from North American ecoregions". Zootaxa Vol 4085, 301-344. DOI: 10.11646/zootaxa.4085.3.1 [Crossref]
33. Powers T.O., Mullin P., Higgins R., Harris T., Powers K.S. (2016b). "Description of Mesocriconema ericaceum n. sp. (Nematoda: Criconematidae) and notes on other nematode species discovered in an ericaceous heath bald community in Great Smoky Mountains National Park, USA". Nematology Vol 18, 879-903. DOI: 10.1163/15685411-00003001 [Crossref]
34. Raski D.J. (1958). "Four new species of Hemicycliophora de Man, 1921, with further observations on H. brevis Thorne, 1955 (Nematoda: Criconematidae)". Proceedings of the Helminthological Society of Washington Vol 25, 125-131.
35. Reed J.P., Jenkins W.R. (1963). "Hemicycliophora vaccinium n. sp. (Nematoda: Criconematidae) from cranberry". Proceedings of the Helminthological Society of Washington Vol 30, 211-212.
36. Ronquist F., Huelsenbeck J.P. (2003). "MRBAYES 3: Bayesian phylogenetic inference under mixed models". Bioinformatics Vol 19, 1572-1574. DOI: 10.1093/bioinformatics/btg180 [Crossref]
37. Schneider W. (1925). "Freilebende Süsswassernematodes aus ostholsteinischen Seen. Nebst Bemerkungen über die Nematodenfauna des Madu und Schaalsees II". Archiv für Hydrobiologie Vol 15, 536-310.
38. Seinhorst J.W. (1962). "On the killing, fixation and transferring to glycerin of nematodes". Nematologica Vol 8, 29-32. DOI: 10.1163/187529262X00981 [Crossref]
39. Seinhorst J.W. (1966). "Killing nematodes for taxonomic study with hot F.A. 4:1". Nematologica Vol 12, 178. DOI: 10.1163/187529266X00239 [Crossref]
40. Subbotin S.A., Vovlas N., Crozzoli R., Sturhan D., Lamberti F., Moens M., Baldwin J.G. (2005). "Phylogeny of Criconematina Siddiqi, 1980 (Nematoda: Tylenchida) based on morphology and D2-D3 expansion segments of the 28S rRNA gene sequences with application of a secondary structure model". Nematology Vol 7, 927-944. DOI: 10.1163/156854105776186307 [Crossref]
41. Subbotin S.A., Sturhan D., Chizhov V.N., Vovlas N., Baldwin J.G. (2006). "Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences". Nematology Vol 8, 455-474. DOI: 10.1163/156854106778493420 [Crossref]
42. Subbotin S.A., Chitambar J.J., Chizhov V.N., Stanley J.D., Inserra R.N., Doucet M.E., McClure M., Ye W., Yeates G.W., Mollov D.S. , et al  (2014). "Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae)". Zoological Journal of the Linnean Society Vol 171, 475-506. DOI: 10.1111/zoj.12145 [Crossref]
43. Swofford D.L. (2003). PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b 10. Sunderland, MA, USA, Sinauer Associates.
44. Tanha Maafi Z., Subbotin S.A., Moens M. (2003). "Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences". Nematology Vol 5, 99-111. DOI: 10.1163/156854102765216731 [Crossref]
45. Tarjan A.C. (1952). "The nematode genus Hemicycliophora de Man, 1921 (Criconematidae) with a description of a new plant-parasitic species". Proceedings of the Helminthological Society of Washington Vol 19, 65-77.
46. Taylor A.L. (1936). "The genera and species of the Criconematidae, a subfamily of the Anguillulinidae (Nematoda)". Transactions of the American Microscopical Society Vol 55, 391-421. [Crossref]
47. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. (1997). "The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools". Nucleic Acids Research Vol 25, 4876-4882. [Crossref]
48. Thorne G. (1955). "Fifteen new species of the genus Hemicycliophora with an emended description of H. typica de Man (Tylenchida, Criconematidae)". Proceedings of the Helminthological Society of Washington Vol 22, 1-16.
49. Van den Berg E. (1981). "Further studies on the genus Hemicycliophora de Man, 1921 in South Africa (Nematoda: Hemicycliophoroidea) with description of a new species". Phytophylactica Vol 13, 181-194.
50. Van den Berg E. (1987a). "New Hemicycliophora species from the Western Cape Province (Hemicycliophoridae: Nematoda)". Phytophylactica Vol 19, 65-70.
51. Van den Berg E. (1987b). "More Hemicycliophora species from South Africa (Hemicycliophoridae: Nematoda)". Phytophylactica Vol 19, 309-314.
52. Van den Berg E., Subbotin S.A., Tiedt L.R. (2010). "Morphological and molecular characterisation of Hemicycliophora lutosa Loof & Heyns, 1969 and H. typica, 1921 from South Africa (Nematoda: Hemicycliophoridae)". Nematology Vol 12, 303-308. DOI: 10.1163/138855409X12511010796671 [Crossref]
53. Van den Berg E., Tiedt L.R., Inserra R.N., Stanley J.D., Vovlas N., Palomares-Rius J.E., Castillo P., Subbotin S.A. (2015). "Characterisation of a topotype and other populations of Hemicriconemoides strictathecatus Esser, 1960 (Nematoda: Criconematidae) from Florida with description of H. phoenicis sp. n. from the USA". Nematology Vol 17, 265-300. DOI: 10.1163/15685411-00002866 [Crossref]
54. Zeng Y., Ye W., Kerns J., Tredway L., Martin S., Martin M. (2015). "Molecular characterization and phylogenetic relationships of plant-parasitic nematodes associated with turf grasses in North Carolina and South Carolina, United States". Plant Disease Vol 99, 982-993. DOI: 10.1094/PDIS-10-14-1060-RE [Crossref]

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Nematology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation