Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Nicotiana benthamiana as model plant for Meloidogyne graminicola infection

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Nematology
For more content, see Nematologica.

Nicotiana benthamiana is widely used as a model plant to analyse cell biology and to obtain insight into the molecular host-pathogen interaction because it is susceptible to many pathogens. Since N. benthamiana can be transformed easily, it is also used to study pathogens for which it is not a known host. Meloidogyne graminicola has a fairly broad host range of mainly monocots and some dicots but no data were available on the ability of M. graminicola to infect N. benthamiana. In this study, we show that M. graminicola is able to infect and complete its life cycle in N. benthamiana, although our experiments demonstrate a lower susceptibility compared to rice. In addition, M. graminicola was also able to develop in N. tabacum but the reproduction was very low. Therefore, we conclude that N. benthamiana can be considered as a host, while this is not the case for N. tabacum.

Affiliations: 1: Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, 9000 Ghent, Belgium

*Corresponding author, e-mail:

Full text loading...


Data & Media loading...

1. Abad P., Favery B., Rosso M.N., Castagnone-Sereno P. (2003). "Root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction". Molecular Plant Pathology Vol 4, 217-224. DOI: 10.1046/j.1364-3703.2003.00170.x [Crossref]
2. Andrieu A., Breitler J., Siré C., Meynard D., Gantet P., Guiderdoni E. (2012). "An in planta, Agrobacterium-mediated transient gene expression method for inducing gene silencing in rice (Oryza sativa L.) leaves". Rice Vol 5, 23. DOI: 10.1186/1939-8433-5-23 [Crossref]
3. Baermann G. (1917). "Eine einfache Methode zur Auffindung von Ankylostomum – (Nematoden) – Larven in Erdproben". In: Mededelingen uit het Geneeskundig Laboratorium te Weltevreden, 41-47.
4. Bellafiore S., Jougla C., Chapuis É., Besnard G., Suong M., Vu P.N., De Waele D., Gantet P., Thi X.N. (2015). "Intraspecific variability of the facultative meiotic parthenogenetic root-knot nematode (Meloidogyne graminicola) from rice fields in Vietnam". Comptes Rendus – Biologies Vol 338, 471-483. DOI: 10.1016/j.crvi.2015.04.002 [Crossref]
5. Bridge J., Plowright R.A., Peng D. (2005). "Nematode parasites of rice". In: Luc M., Sikora R.A., Bridge J. (Eds). Plant parasitic nematodes in subtropical and tropical agriculture, 2nd edition. Wallingford, UK, CAB International, pp.  87-130. [Crossref]
6. Castagnone-Sereno P. (2002). "Genetic variability of nematodes: a threat to the durability of plant resistance genes?" Euphytica Vol 124, 193-199. DOI: 10.1023/A:1015682500495 [Crossref]
7. Chapuis E., Besnard G., Andrianasetra S., Rakotomalala M., Nguyen H.T., Bellafiore S. (2016). "First report of the root-knot nematode (Meloidogyne graminicola) in Madagascar rice fields". Australasian Plant Disease Notes Vol 11, 32. DOI: 10.1007/s13314-016-0222-5 [Crossref]
8. Chen J., Lin B., Huang Q., Hu L., Zhuo K., Liao J. (2017). "A novel Meloidogyne graminicola effector, MgGPP, is secreted into host cells and undergoes glycosylation in concert with proteolysis to suppress plant defenses and promote parasitism". PLoS Pathogens Vol 13. DOI: 10.1371/journal.ppat.1006301
9. Davarpanah S.J., Jung S.H., Kim Y.J., Park Y.I., Min S.R., Liu J.R., Jeong W.J. (2009). "Stable plastid transformation in Nicotiana benthamiana". Journal of Plant Biology Vol 52, 244-250. DOI: 10.1007/s12374-009-9023-0 [Crossref]
10. Fanelli E., Cotroneo A., Carisio L., Troccoli A., Grosso S., Boero C., Capriglia F., De Luca F. (2017). "Detection and molecular characterization of the rice root-knot nematode Meloidogyne graminicola in Italy". European Journal of Plant Pathology Vol 149, 467-476. DOI: 10.1007/s10658-017-1196-7 [Crossref]
11. Golden A.M., Birchfield W. (1965). "Meloidogyne graminicola (Heteroderidae) a new species of root-knot nematode from grass". Proceedings of the Helminthological Society of Washington Vol 32, 228-231.
12. Goodin M.M., Zaitlin D., Naidoo R.A., Lommel S.A. (2008). "Nicotiana benthamiana: its history and future as a model for plant-pathogen interactions". Molecular Plant-Microbe Interactions Vol 21, 1015-1026. DOI: 10.1094/MPMI-21-8-1015 [Crossref]
13. Haegeman A., Mantelin S., Jones J.T., Gheysen G. (2012). "Functional roles of effectors of plant-parasitic nematodes". Gene Vol 492, 19-31. DOI: 10.1016/j.gene.2011.10.040 [Crossref]
14. Izawa T., Shimamoto K. (1996). "Becoming a model plant: the importance of rice to plant science". Trends in Plant Science Vol 1, 95-99. DOI: 10.1016/S1360-1385(96)80041-0 [Crossref]
15. Jenkins W.R. (1964). "A rapid centrifugal-flotation technique for separating nematodes from soil". Plant Disease Reporter Vol 48, 692.
16. Kapila J., De Rycke R., Van Montagu M., Angenon G. (1997). "An Agrobacterium-mediated transient gene expression system for intact leaves". Plant Science Vol 122, 101-108. DOI: 10.1016/S0168-9452(96)04541-4 [Crossref]
17. Kyndt T., Fernandez D., Gheysen G. (2014). "Plant-parasitic nematode infections in rice: molecular and cellular insights". Annual Review of Phytopathology Vol 52, 135-153. DOI: 10.1146/annurev-phyto-102313-050111 [Crossref]
18. Li Y., Wang K., Lu Q., Du J., Wang Z., Wang D., Sun B., Li H. (2017). "Transgenic Nicotiana benthamiana plants expressing a hairpin RNAi construct of a nematode Rs-cps gene exhibit enhanced resistance to Radopholus similis". Scientific Reports Vol 7, 13126. DOI: 10.1038/s41598-017-13024-9
19. Liu W., Wang G.L. (2016). "Plant innate immunity in rice: a defense against pathogen infection". National Science Review Vol 3, 295-308. DOI: 10.1093/nsr/nww015 [Crossref]
20. Ma L., Lukasik E., Gawehns F., Takken F.L.W. (2012). "The use of agroinfiltration for transient expression of plant resistance and fungal effector proteins in Nicotiana benthamiana leaves". In: Bolton M.D., Thomma B.P. (Eds). Plant fungal pathogens. Methods in molecular biology (methods and protocols) 835. New York, NY, USA, Humana Press, pp.  61-74. DOI: 10.1007/978-1-61779-501-5_4 [Crossref]
21. Mantelin S., Bellafiore S., Kyndt T. (2017). "Pathogen profile. Meloidogyne graminicola: a major threat to rice agriculture". Molecular Plant Pathology Vol 18, 3-15. DOI: 10.1111/mpp.12394 [Crossref]
22. Nguyen H.P., Chakravarthy S., Velásquez A.C., McLane H.L., Zeng L., Nakayashiki H., Park D.H., Collmer A., Martin G.B. (2010). "Methods to study PAMP-triggered immunity using tomato and Nicotiana benthamiana". Molecular Plant-Microbe Interactions Vol 23, 991-999. DOI: 10.1094/MPMI-23-8-0991 [Crossref]
23. Nguyễn P.V., Bellafiore S., Petitot A., Haidar R., Bak A., Abed A., Gantet P., Mezzalira I., Engler J.D.A., Fernandez D. (2014). "Meloidogyne incognita-rice (Oryza sativa) interaction: a new model system to study plant-root-knot nematode interactions in monocotyledons". Rice Vol 7, 23. DOI: 10.1186/s12284-014-0023-4 [Crossref]
24. Perry R.N. (1997). "Plant signals in nematode hatching and attraction". In: Fenoll C., Grundler F.M.W., Ohl S.A. (Eds). Cellular and molecular aspects of plant-nematode interactions. Developments in plant pathology 10. Dordrecht, The Netherlands, Springer, pp.  38-50. DOI: 10.1007/978-94-011-5596-0_4 [Crossref]
25. Pokharel R.R., Abawi G.S., Zhang N., Duxbury J.M., Smart C.D. (2007). "Characterization of isolates of Meloidogyne from rice-wheat production fields in Nepal". Journal of Nematology Vol 39, 221-230.
26. Pokharel R.R., Abawi G.S., Duxbury J.M., Smat C.D., Wang X., Brito J.A. (2010). "Variability and the recognition of two races in Meloidogyne graminicola". Australasian Plant Pathology Vol 39, 326-333. DOI: 10.1071/AP09100 [Crossref]
27. Postma W.J., Slootweg E.J., Rehman S., Finkers-Tomczak A., Tytgat T.O., van Gelderen K., Lozano-Torres J.L., Roosien J., Pomp R., van Schaik C. , et al (2012). "The effector SPRYSEC-19 of Globodera rostochiensis suppresses CC-NB-LRR-mediated disease resistance in plants". Plant Physiology Vol 160, 944-954. DOI: 10.1104/pp.112.200188 [Crossref]
28. Prasad J.S., Panwar M.S., Rao Y.S. (1984). "Studies on the control of Meloidogyne graminicola on rice". Nematologia Mediterranea Vol 12, 141-143.
29. Ramegowda V., Senthil-Kumar M. (2015). "The interactive effects of simultaneous biotic and abiotic stresses on plants: mechanistic understanding from drought and pathogen combination". Journal of Plant Physiology Vol 176, 47-54. DOI: 10.1016/j.jplph.2014.11.008 [Crossref]
30. Reversat G., Boyer J. (1999). "A mixture of sand and water-absorbent synthetic polymer as substrate for the xenic culturing". Nematology Vol 1, 209-212. DOI: 10.1163/156854199508027 [Crossref]
31. Reynolds A.M., Dutta T.K., Curtis R.H.C., Powers S.J., Gaur H.S., Kerry B.R. (2011). "Chemotaxis can take plant-parasitic nematodes to the source of a chemo-attractant via the shortest possible routes". Journal of the Royal Society, Interface Vol 8, 568-577. DOI: 10.1098/rsif.2010.0417 [Crossref]
32. Song G., Yamaguchi K. (2003). "Efficient agroinfiltration-mediated transient GUS expression system for assaying different promoters in rice". Plant Biotechnology Vol 20, 235-239. DOI: 10.5511/plantbiotechnology.20.235 [Crossref]
33. Yik C., Birchfield W. (1979). "Host studies and reactions of rice cultivars to Meloidogyne graminicola". Phytopathology Vol 69, 497-499. DOI: 10.1094/Phyto-69-497 [Crossref]
34. Yousif G.M. (1979). "Histological responses of four leguminous crops infected with Meloidogyne incognita". Journal of Nematology Vol 11, 395-401.
35. Zhang B., Yang Y., Wang J., Ling X., Hu Z., Liu T., Chen T., Zhang W. (2015). "A CC-NBS-LRR type gene GHNTR1 confers resistance to southern root-knot nematode in Nicotiana benthamiana and Nicotiana tabacum". European Journal of Plant Pathology Vol 142, 715-729. DOI: 10.1007/s10658-015-0646-3 [Crossref]

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Nematology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation