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Characterisation of biological and biocontrol traits of entomopathogenic nematodes promising for control of striped flea beetle (Phyllotreta striolata)

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The biological and biocontrol traits of two entomopathogenic nematode isolates, Steinernema pakistanense 94-1 (Sp94-1) and Heterorhabditis indica 212-2 (Hi212-2), were evaluated. The highest yield of infective juveniles (IJ) in monoxenic sponge culture system for Sp94-1 and Hi212-2 was 3.52 (± 0.45) × 105 and 7.08 (± 0.11) × 105 IJ g−1, respectively. The optimum storage temperature was 25°C for Sp94-1 and 14°C for Hi212-2. Sp94-1 showed greater tolerance to heat exposure and UV radiation, while S. carpocapsae All, a commercial strain, was more resistant to osmotic pressure, desiccation, cold treatment and hypoxia than the other tested isolates. Hi212-2 suppressed the Phyllotreta striolata larvae when applied at 1.5 × 109 IJ ha−1 or higher concentrations, while Sp94-1 suppressed the P. striolata larvae only when applied at 4.5 × 109 IJ ha−1. Our study indicates the possibility of commercialisation of the EPN isolates, and further confirms their efficacy against the P. striolata larvae in the field.

Affiliations: 1: 1Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, No. 105 Xingang Road West, Guangzhou, P.R. China ; 2: 2Foshan Xiaonong Eco-Technology Co., Ltd, Nanfeng Road, Leping county, Sanshui District, Foshan, P.R. China

*Corresponding author, e-mail:

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