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The use of Pluronic F-127 to study the development of the potato cyst nematode, Globodera pallida

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image of Nematology
For more content, see Nematologica.

This paper demonstrates a simple novel in vitro method using Pluronic F-127 aqueous solution to study the development of the potato cyst nematode, Globodera pallida, in Solanum spp. without any need for sterilisation of either the plants or the nematodes. In this study, this method was successfully applied to comparative studies on the development of G. pallida in Solanum tuberosum (potato) or S. sisymbriifolium (sticky nightshade). The protocol described here could be useful for screening transgenic plants or different plant cultivars/species for their ability to allow development not only of G. pallida but also any other plant-parasitic nematodes.

Affiliations: 1: 1Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK; 2: 3Branston Ltd, Mere Road, Branston, Lincoln LN4 1NJ, UK; 3: 2The University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK


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