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Production of species-specific Meloidogyne populations for the identification of resistance in germplasm collections

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Field populations sometimes are a mixture of species and genotypes, which makes them unsuitable for resistance screening against root-knot nematodes. To solve this problem, a special protocol was developed to produce species-specific populations originating from single juveniles and to prevent contamination of inoculum of species-specific populations by other aggressive Meloidogyne species. These populations are representative of the type races occurring in the Netherlands. Before multiplication of the egg mass, the Meloidogyne female was identified by isozyme patterns of esterase (Est) and malate dehydrogenase (Mdh). The Petri dish method was evaluated to study the infection process of M. hapla, M. chitwoodi, and M. fallax on potato roots. High infection levels were obtained following inoculation with a water suspension of juveniles. The potential use of the Petri dish method for screening for resistance was assessed by inoculation of juveniles of Globodera species on roots of susceptible and resistant potato cultivars, resulting in clear differential reactions. During this study, a deviating group of field populations was distinguished based on isozyme band patterns. It was later described as a new species, M. fallax.

Production de populations de Meloidogyne specifiques de l'espece en vue de l'identification de la resistance dans les collections de germplasmes - Les population des Meloidogyne rencontrees au champ peuvent etre des melanges d'especes et de genotypes et de ce fait ne conviennent pas pour tester la resistance a ces nematodes. Afin de surmonter cette difficulte, un protocole particulier a ete mis au point visant, a partir d'un seul juvenile, a produire des populations specifiques de l'espece et a eviter la contamination de ces populations par d'autres especes agressives de Meloidogyne. Ces populations sont representatives des races types presentes aux Pays Bas. En prealable a la multiplication de la masse d'oeufs, il a ete realise une pre-identification de la femelle a l'aide de profils esterasiques concernant l'esterase (Est) et la malate deshydrogenase (Mdh). La methode dite des boites de Petri a ete testee en vue de l'etude du processus d'infestation de M. hapla, M. chitwoodi et M. fallax sur des racines de pomme de terre. Grace a ce systeme, des taux d'infestation eleves sont obtenus apres inoculation a l'aide d'une suspension de juveniles dans l'eau. Les potentialites de la methode des boites de Petri ont ete confirmees par inoculation de juveniles de Globodera dans des racines de cultivars de pomme de terre sensibles et resistants, lesquels ont montre des reactions tres differentes. Au cours de ces recherches, un groupe aberrant de populations sauvages a pu etre distingue grace aux profils des isozymes, groupe decrit ensuite comme une espece nouvelle, M. fallax.

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/content/journals/10.1163/156854199507956
1999-02-01
2016-12-08

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