Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Identification of potato cyst nematode species Globodera rostochiensis and G. pallida by PCR using specific primer combinations

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Nematology
For more content, see Nematologica.

RAPD fragments specific for potato cyst nematode (PCN) species Globodera rostochiensis and G. pallida were identified. These were used to derive two primer combinations for PCR which amplify only one specific band in each species. The primer combinations were evaluated on 39 samples of PCN populations containing all currently described pathotypes as well as some South American PCN populations. This method for species identification is highly sensitive and also allows the detection of species mixtures in a sample. Furthermore, the generation of unique amplification products facilitates the application of automatic detection methods without electrophoretic separation and is therefore particularly useful for routine diagnoses.

Identification des especes de nematodes a kyste de la pomme de terre Globodera rostochiensis et G. pallida par PCR grace a l'utilisation de combinaisons d'amorces specifiques - Des fragments RAPD specifiques des especes de nematodes a kyste de la pomme de terre (PCN) Globodera rostochiensis et G. pallida, ont ete identifies. A partir de ces fragments, deux combinaisons d'amorces, capables d'amplifier en PCR une seule bande, specifique pour chaque espece, ont ete obtenus. Les combinaisons d'amorces ont ete evaluees sur 39 echantillons de populations de PCN contenant tous les pathotypes decrits ainsi que quelques populations provenant d'Amerique du Sud. Cette methode d'identification d'espece est tres sensible et elle permet de plus l'identification d'un melange d'especes dans un meme echantillon. En sus, la generation de produits uniques d'amplification permet l'utilisation de methodes d'identification automatisees, sans separation electrophoretique, tres utiles donc pour le diagnostic de routine.


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Nematology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation