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Meloidogyne chitwoodi and M. fallax protein variation assessed by two-dimensional electrophoregram computed analysis

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The variation of total soluble protein extracts from white adult females of seven Meloidogyne chitwoodi and three M. fallax isolates were studied using two dimensional gel electrophoresis (2-DGE). The 2-D protein profiles were compared with a computed analysis system. A synthetic image or 'master' was constructed from proteinic spots present in each isolate. The comparison of the two species allowed detection of qualitative and, to a lesser extent, quantitative variations. Among the 243 proteinic spots present on the master, we have identified 75 monomorphic spots for both species and nine discriminative proteins for each of the two species. In addition, two isoelectric point variants and two quantitative variants were observed. The similarity index and the genetic distances between the ten isolates were calculated on the basis of homologous polymorphic spots. Dendrograms were constructed according to the UPGMA method. Significance of the results from the phenetic study was assessed by bootstrap analysis. The results from the clustering analysis allowed differentiation between both species and showed intraspecific variation among the M. chitwoodi isolates equal to that among the M. fallax isolates. Variabilite proteique de Meloidogyne chitwoodi et M. fallax etudiee par analyse informatisee d'electrophoregrammes bidimensionnels - La variabilite d'extraits proteiques solubles totaux de femelles blanches adultes de sept isolats de Meloidogyne chitwoodi et trois de M. fallax a ete etudiee par electrophorese sur gel en deux dimensions (2-DGE). Les profils proteiques bidimensionels ont ete compares grace a un systeme d'analyse informatise. Une image synthetique, ou image de reference, a ete construite a partir des spots proteiques toujours observes dans chaque isolat. La comparaison des deux especes a permis de detecter des variations qualitatives et, dans une moindre mesure, quantitatives. Parmi les 243 taches proteiques presentes sur l'image de reference, ont ete identifies 75 polypeptides communs aux deux especes et neuf proteines discriminantes pour chacune des deux especes. De plus, deux variants de point isoelectrique et deux variants quantitatifs ont ete observes. Les indices de similarite et les distances genetiques entre les dix isolats ont ete etudies a partir des taches polymorphes homologues. Les dendrogrammes ont ete construits selon la methode UPGMA. La signification des resultats phenetiques a ete estimee par une analyse "bootstrap". Les resultats de l'analyse en grappes ont permis de differencier les deux especes et ont revele une variabilite intraspecifique similaire entre isolats de M. chitwoodi et isolats de M. fallax.


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