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Effects of surface sterilisation and cold storage on in vitro behaviour of Pratylenchus penetrans

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For more content, see Nematologica.

0.1% malachite green alone (15 min) or with 0.5% streptomycin sulphate were efficient to surface sterilise Pratylenchus penetrans. These treatments did not significantly reduce nematode movement, nor attraction to and penetration into Rosa dumetorum cv. Laxa seedlings. Streptomycin sulphate (0.2%, 24 h) and a split treatment of streptomycin sulphate (0.2%, 24 h) and malachite green (0.1%, 10 min) did not reduce surface contamination. Combinations based on mercuric chloride (0.05-0.1%, 1-1.5 min) affected the behaviour of juvenile and adult stages of P. penetrans. Storage (30 days) at 4 degrees C reduced the survival of P. penetrans and its attraction to and penetration into rose seedlings.

Effets de la sterilisation superficielle et du stockage au froid sur le comportement in vitro de Pratylenchus penetrans - Un traitement de 15 min a l'aide de vert de malachite a 0,1%, seul ou additionne de sulfate de streptomycine a 0,5%, sterilise superficiellement Pratylenchus penetrans de facon efficace. De tels traitements ne diminuent pas les mouvements du nematode non plus que son attraction par et sa penetration dans les racines de plants de Rosa dumetorum cv. Laxa. L'utilisation de sulfate de streptomycine a 0,2% pendant 24 h ou un traitement en deux temps par le sulfate de streptomycine (0,2%; 24 h) puis par le vert de malachite (0,1%; 10 min) ne diminuent pas la contamination superficielle. Les traitements combines comprenant du chlorure de mercure (0,05-0,1%; 1-5 min) affectent le comportement des juveniles et des adultes de P. penetrans. Un stockage de 30 jours a 4 degrees C diminue la survie de P. penetrans ainsi que ses potentialites d'attraction et de penetration vis-a-vis des plants de rosier.


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