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Immunocytochemical Localization of Gonadotropin and Gonadotropin Releasing Hormone in Pituitary and Brain of the Gilthead Seabream, Sparusaurata L. (Teleost)

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image of Netherlands Journal of Zoology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Animal Biology (Vol 53 and onwards).

Characterization of gonadotropic cells in the pituitary gland of male gilthead seabream (Sparus aurata) was performed at the light- and electron microscopic level with the peroxidase-anti peroxidase and the immunogold technique, respectively. The cells were identified with antibodies raised against three different teleost gonadotropins (carp α, β-GTH, carp β-GTH, catfish α, β-GTH). Immunostained cells were mainly located in the ventral and dorsal areas of the proximal pars distalis (PPD), with a few cells in the pars intermedia (PI). At the light microscopic level the gonadotrops showed a variable granulation and they were often strongly vacuolated. In the electron microscope the latter appeared to be caused by strongly dilated rough endoplasmic reticulum (RER). Immunogold labeling was restricted to all secretory granules and most of the larger globules. Different fixation and embedding procedures were tested: an optimal combination of good ultrastructure and intense immunogold labeling was obtained when osmium tetroxide postfixation was omitted and with embedding in 'Epon. The distribution of gonadotropin-releasing hormone (GnRH) immunoreactivity was studied with antibodies against mammalian LHRH and salmon GnRH. Only with anti-mammalian LHRH immunostained perikarya were observed in the ventral preoptic area. With both antisera immunostained fibers were seen directed towards the pituitary stalk, as well as in the neurohypophysis, whereas no such fibers were present in the PPD. Immunostained fibers were also found in several other regions of the brain.

Affiliations: 1: Department of Cell Biology, Faculty of Biology, University of Murcia, 30071 Murcia, Spain; 2: Department of Experimental Zoology, Research Group for Comparative Endocrinology, State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands


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