Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Putative No-Synthesizing Neurons of Lymnaea in Vivo and in Vitro

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Netherlands Journal of Zoology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Animal Biology (Vol 53 and onwards).

The distribution of putative nitric oxide synthase- (NOS) containing neurons in the CNS and peripheral tissues of the freshwater pulmonate molluscs, Lymnaea stagnalis, Helisoma trivolvis and Biomphalaria sp. was investigated using NADPH-diaphorase (NADPH-d) histochemistry and NOS-immunolabelling. NADPH-d-positive cells were found in most of the central ganglia but their distribution was different from that of other ncurotransmitters. Comparison of NADPH-d-positive neurons in the CNS of Lymnaea, Helisoma and Biomphalaria showed that bulinid snails contained more NADPH-d positive neurons in the CNS, especially in the cerebral ganglia, compared to Lymnaea. Some of previously identified neurons were found to be NADPH-d-positive (e.g. a buccal gut motoneuron B2 in Lymnaea and its homologues B5 in the bulinids). In addition, the right parietal light yellow' cluster cells (LYC) were also weakly stained in Lymnaea both with NOS antibodies and NADPH-d. Neurons B2 and LYC were isolated and maintained in culture under conditions that support neurite outgrowth. In culture, both B2 and LYC were stained by NADPH-d similar to that observed in whole mount preparations. In addition to cell somata, intense NADPH-d positive staining was observed in the growth cone and at the tips of filopodia of B2 neurons. We suggest that NO (or related substances) is involved in neuronal signalling in molluscan species.

Affiliations: 1: Department of Physiology, Byelorussian State University, Minsk, 220080, Republic of Belarus, Neuroscience and Respiratory Research Groups, Faculty of Medicine, The University of Calgary, HSC, 3330 Hospital Drive, NW, Calgary, AB, Canada T2N 4N1; 2: Neuroscience and Respiratory Research Groups, Faculty of Medicine, The University of Calgary, HSC, 3330 Hospital Drive, NW, Calgary, AB, Canada T2N 4N1


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Netherlands Journal of Zoology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation