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Occurrence of an Ovarian Opioid System in Oviparous Vertebrates: Proopiomelanocortin mRna Expression in the Ovary of the Green Water Frog, Rana Esculenta

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image of Netherlands Journal of Zoology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Animal Biology (Vol 53 and onwards).

The aim of the present study was to investigate whether the proopiomelanocortin (POMC) gene is expressed in the ovary of Rana esculenta. Using a homologous probe, the presence of POMC mRNA could not be detected by Northern blot analysis in the ovary, while a positive signal was found in the pituitary gland. In contrast, after amplification of ovarian mRNA by the PCR technique, an intense signal of 480 bp was detected by Southern blotting, indicating that POMC is actually synthesized in the ovary.

Affiliations: 1: Dipartimento di Biologia (MCA), Università di Camerino, via F. Camerini 2, 62032 Camerino (MC), Italy; 2: European Institute for Peptide Research, Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U413d, UA CNRS, University of Rouen, 76821 Mont-Saint-Aignan, France

10.1163/156854295X00834
/content/journals/10.1163/156854295x00834
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/content/journals/10.1163/156854295x00834
1994-01-01
2016-12-10

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