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Cytoskeletai, Proteins and Steroidogenesis in Granulosa and Theca Cells of Porcine Follicle

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image of Netherlands Journal of Zoology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Animal Biology (Vol 53 and onwards).

The participation of microtubules and microfilaments in steroidogenesis of both theca (T) and granulosa (G) cells in mono- and coculture was assayed by monitoring the effect of colchicine (Col) and cytochalasin B (Cyt B) on estradiol (E2) secretion during 24 hours of culture. In coculture Col and Cyt stimulated E2 secretion, which suggests that both microtubules and microfilamentes are involved in E2 secretion.

Affiliations: 1: Laboratory of Animal Endocrinology and Tissue Culture, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, 30-060 Krakow, Ingardena 6, Poland


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