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Continuous surface modification of silica particles for enzyme immobilization

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A novel reaction process for surface modification of ceramic particles is investigated for enzyme immobilization. The surface modification of the support particles can be carried out continuously in a flow reactor. The proposed continuous surface modification process (CSMP) is advantageous for the handling of particles in the catalyst preparation process. Using this reaction method, the surface of silica particles was easily modified for enzyme immobilization. The degree of surface modification could be controlled by the reaction conditions and the amount of immobilized enzyme was also controlled. Lysozyme and catalase were immobilized on the silica particles modified by the CSMP method. The adsorption of lysozyme was studied as an example of enzyme adsorption on the modified particles. The enzyme activity of prepared particles was tested for decomposition of H2O2 by immobilized catalase. It was shown that the obtained particles indicated the enzyme activity and CSMP is a useful method for preparing enzyme catalysts.


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