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Effect of monodispersed silica nanoparticles on DNA separation by micro-capillary electrophoresis

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In this work, we investigate micro-capillary electrophoresis (μ-CE) using monodispersed SiO2 nanoparticles added to an electrophoresis buffer solution to obtain high mobility and high separation of double-strand DNA. Various particle sizes of monodispersed SiO2 nanoparticle solutions were mixed with conventional 0.7% hydroxyl propyl methyl cellulose (HPMC) buffer solution to achieve μ-CE. We achieved perfect separation of the DNA specimen (100 bp to 1.5 kbp) at a certain SiO2 nanoparticle size. In addition, we discuss the effect of SiO2 nanoparticles during electrophoresis.


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