Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Microbiochemical reactors for enzymatic reactions including cell-free mRNA translation

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

This Article is currently unavailable for purchase.
Add to Favorites
You must be logged in to use this functionality

As the first step toward the 'lab on a chip' or 'cell on a chip' concept, several microbiochemical reactors were fabricated using conventional MEMS (microelectromechanical systems) techniques: silicon anisotropic etching and glass-silicon anodic bonding. The microreactors have main reaction channels which are 14 mm long, 200-800 μm wide, and 20 μm deep. In these channels, enzymatic reactions - luciferin-luciferase reaction and cell-free mRNA translation - were demonstrated. Luminescence induced by the luciferin-luciferase reaction was observed in two ways, by photograph on a high-speed film and sensitive video imaging. In both cases, diffusion-based mixing in the microreactors was clearly visualized. In the cell-free protein synthesis experiment, the amount of polyphenylalanine, which was synthesized by translating polyuridylic acid template in the microreactors, was determined using radioisotope assay.

Affiliations: 1: Biochemical Systems Laboratory, The Institute of Physical and Chemical Research (RIKEN), 2-1, Hirosawa, Wako-shi, Saitama 351-0198, Japan; 2: Department of Electronics, Information and Communication Engineering, Waseda University, 3-4-1, Ohkubo, Shinjuku-ku, Tokyo 169-0072, Japan


Full text loading...


Data & Media loading...

Article metrics loading...



Can't access your account?
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation