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The Esr Study On the Protective Effect of Grape Seed Extract On Rat Heart Mitochondria From the Injury of Lipid Peroxidation

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The effect of grape seed extract (GSE) on the lipid peroxidation of rat heart mitochondria was studied using the spin trapping agent α-(4-pyridyl-1-oxide)-N-t-butylnitrone (4-POBN) with electron spin resonance (ESR) spectrometer. Two fatty acid spin labels, 5-doxyl-stearic acid (5-DOXYL) and 16-doxyl-stearic acid (16-DOXYL) were used to study the effect of GSE on mitochondrial membrane fluidity. The results showed that the GSE could scavenge the lipid free radicals generated from the mitochondria of rat heart, and protect the mitochondrial membranes damaged by lipid peroxidation. Both the scavenging effect and the protective effect are better in water phase than in lipid phase. And the data indicate that perhaps trimers and other polymers have better scavenging effect on lipid free radicals than monomers.

Affiliations: 1: Laboratory of Visual Information Processing, Department of Molecular and Cell Biophysics, Institute of Biophysics, Academia Sinica, Beijing 100101, P.R.CHINA; 2: Institute of Food Research, Norwich Research Park, Norwich NR4 7NA, UNITED KINGDOM

10.1163/156856700X00723
/content/journals/10.1163/156856700x00723
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/content/journals/10.1163/156856700x00723
2017-10-19

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