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Ftir Study of the Protonation of a Retinyl Schiff Base in Chloroform/Methanol Mixtures

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Fourier transform infrared spectra of a retinyl Schiff base in the presence of one of a series of acids were recorded in solvents containing chloroform and methanol in different proportions. The spectra show that methanol makes it possible for weak acids to achieve complete protonation of the Schiff base. This leads to the hypothesis that water or other OH containing molecules could play a role in bringing about complete protonation of the Schiff base chromophore in rhodopsins.

Affiliations: 1: Département de Chimie, Université du Québec à Montreal C.P. 8888, Succ. A Montreal, Québec, Canada H3C 3P8; 2: Département de Chimie, Université de Montréal C.P. 6128, Succ. A Montreal, Québec, Canada H3C 3J7


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