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Molecular Variability of Lignin Fractions Isolated From Wheat Straw

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Three lignin fractions were isolated from wheat straw internodes and leaves: alkali lignin (LA), milled lignin (LM) and enzyme lignin (LE). Thioacidolysis and alkaline nitrobenzene oxidation were used for the characterisation of the lignin monomeric composition. Esterified and etherifiedp-coumaric and ferulic acids were characterised by alkali hydrolysis and acidolysis, respectively. Lignin fractions from leaves are more condensed than those from internodes. The syringyl/guaiacyl (S/G) ratio is higher in the case of internode lignins. Among the three lignin fractions, LA is the less condensed whereas LM and LE do not present significant structural differences according to their monomeric composition. Finally, the lignin fractions studied differ in their contents and proportions of associated phenolic acids.

Affiliations: 1: Laboratoire de Chimie Biologique, INRA, InstitutNational Agronomique Paris-Grignon, 78850 THIVERYAL-GRIGNON, FRANCE


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