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Molecular Organization of Dhp Membrane Fragments

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The phase transition of dihexadecyl phosphate (DHP) bilayered disks has been studied using EPR spectroscopy. In the acid form of DHP, a phase transition temperature exists, that we have monitored through the spin-spin interaction between the nitroxide molecules at high concentration (8%) in DHP bilayers. This spin-spin interaction is due to the gathering of solutes in a fluid defect of the membrane: the border. The fluorescence quenching of two probes by the nitroxide stearic acids in DHP bilayers has been studied by stationary and time-resolved fluorescence measurements. The quenching process is mainly static. Both magnetic and fluorescent probes are localized in the periphery of the bilayered disks.

Affiliations: 1: Equipe ENS, Physico-Chimie des Rayonnements, CNRS URA75, Université Paris Sud, 91405 Orsay Cedex, France; 2: Bioénergétique membranaire, CNRS URA1128, Université Paris Sud, 91405 Orsay Cedex, France; 3: Photophysique et Photochimie Moléculaire, CNRS URA348, Université Bordeaux I, 351, Cours de la Libération, 33405 Talence, France


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