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Chlorophyllin Coupled With Polyethylene Glycol: . a Potent Photosensitizer

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Chlorophyllin a prepared by the saponification of chlorophyll a was conjugated with α-(3-aminopropyl)-ω-methoxypoly(oxyethylene), PEG-NH2, to form PEG-chlorophyllin through acid-amide bonds. PEG-chlorophyllin was readily soluble not only in aqueous solution but also in organic solvents. The absorption maxima of the conjugate were located at 657 nm and 418 nm in aqueous solution, and at 656 nm and 422 nm in benzene. The conjugate seems to be more stable toward light-illumination than uncoupled chlorophyllin. Furthermore, PEG-chlorophyllin possessed a potent photosensitizing activity to produce superoxide anion during the illumination, although chlorophyllin immediately lost its activity by light-illumination.

Affiliations: 1: Toin Human Science and Technology Centre, Department of Materials Science and Technology, Toin University of Yokohama, Kurogane-cho, Aoba-ku, Yokohama, 225 JAPAN


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