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Extraction and determination of chlorophylls from moso bamboo (Phyllostachys pubescens) culm

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The purpose of this study is to establish a simple and reliable method for bamboo chlorophyll extraction. Chlorophylls in moso bamboo (Phyllostachys pubescens) epidermis were extracted with acetone, DMF (N,N-dimethylformamide) and DMSO (dimethyl sulfoxide) using three methods, including ultrasonics, centrifugation and grinding. Ultraviolet-visible spectrometry was then used to evaluate the efficiency of these extraction methods. It was also used to quantitatively analyze the extracted chlorophylls. The results revealed that the extraction efficiency of epidermis chlorophylls is related to the size of bamboo culm meal, solvent types, and the method of extraction and filtration. A fast and reliable extraction method was developed. It extracts chlorophylls from bamboo culm meal (<0.7 mm) using an acetone bath in ultrasonics for 3 min and followed by centrifugal filtration. This extraction procedure has been proven to be easy to use and also highly reproducible. Chlorophylls in acetone showed the best stability, followed by DMF and then DMSO. In a dark environment kept at 4°C, chlorophylls can be preserved for up to 8 days in DMF and 30 days in acetone. On the other hand, acetone extracts higher content of chlorophylls. In 80% acetone, DMF and DMSO extracts, the total chlorophylls contents are 4.80, 4.18, and 3.78 mg per gram of epidermis meal, respectively.


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