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Adhesion strength of various mammalian cell lines, Part I: Effect of the serum origin and its concentrations

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Adhesion strength of L929 and Hela B cells in term of critical shear stress of detachment in response to various concentrations of serum was measured. It is found that when the concentration of serum in the culture medium sequentially decreased from 0.25% to 0.1%, L929 cells lost their adhesion strength gradually and at or <0.05% cells could not survived whereas Hela B cells for their adhesion strength were found more dependent on serum. They lost of adhesion strength at 1.25% serum and could not survived at 0.25% serum containing culture medium. When the medium was supplemented with a good growth serum free medium (Nutridoma) both the cell lines showed their basal adhesion strength value. Unlike L929 and Hela B cells, Walker rat carcinoma cells showed a significant increase (95%) in their adhesion strength in response to fetal calf serum as compared to horse serum.


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