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Adhesion strength of various mammalian cell lines, Part II: Effect of polystyrene, glass and ageing on various mammalian cell lines

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image of Biogenic Amines

In this study, adhesion strength of various mammalian cell lines on polystyrene has been quantitatively determined and compared with that of a glass substratum. Six cell lines were tested. All the cell lines except CHL cells stuck better to polystyrene than to a glass substratum. MDCK cells were found more adhesive and Walker rat carcinoma cells least adhesive. It has been found that every cell line possessed a specific and constant adhesion strength value. Some cell lines like BHK cells showed age dependent decline in adhesion strength, whereas Hela B and L929 cells did not exhibit this phenomena; instead they retained their specific adhesion strength value even after 24 weeks. In contrast, BHK cells were able to retain only 13% of the original adhesion strength value at the end of the 24th week of their ageing. The results of this study show that every cell line has its own mechanism for attaining the final adhesion strength. These results can be applied both in biotechnology and in basic research in biological sciences.


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