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Black mulberries (Morus nigra) as a natural dye for animal tissues staining

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image of Animal Biology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Netherlands Journal of Zoology (Vol 18-52).

Natural dyes produce an extraordinary diversity of rich and complex colours as well as unexpected results, making them exciting to use. Natural dyes have been used for staining wool, silk, carpet and cotton. Black mulberry (Morus nigra) has strong staining activity and a distinct flavor with juicy and acidic characteristics making them attractive for use in the processing industry in products such as fruit juice, ice cream, jelly, and jam. Aim of this study was to investigate a new staining method using black mulberry for whole mount and transverse sections staining of fasciola. Adult liver flukes (Fasciola sp.) were collected from the livers of naturally infected cows at slaughterhouse, washed with physiological saline solution. Some adult Fasciola were collected, immersed in 10% neutral buffered formalin for fixation, and embedded in paraffin for histological studies. The rest of whole mount of adult worms were collected, and then stained by the new method (dye extracted form beet root) and Carmine staining method for control. Sections, 7-10 micrometer from adult worms were collected, and then stained by the new method and hematoxyllin & eosin staining method for control. By using the dye extracted from beet root, zoologists and parasitologists can make identification and differentiation between different parasites. By using the dye extracted from black mulberry, zoologists and parasitologists can make identification and differentiation between different parasites. This dye method can be an alternative to cost and time consuming current chemical staining methods.

Affiliations: 1: Department of Zoology, Faculty of Science, Tanta University, Egypt; 2: Science Department, College of Basic Education, PAAET, Kuwait

10.1163/157075511X554419
/content/journals/10.1163/157075511x554419
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/content/journals/10.1163/157075511x554419
2011-03-01
2017-09-21

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