Cookies Policy
X

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Fasting and postprandial conditions affect both fatty acids and lipid compositions in the hypothalamus and fat-soluble vitamins in the serum of male rats

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Animal Biology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Netherlands Journal of Zoology (Vol 18-52).

The purpose of this study was to examine the effects of fasting and re-feeding on lipid derivative patterns in the hypothalamus, and on vitamin, cholesterol and malondialdehyde (MDA) levels in serum. Adult male Wistar albino rats were assigned (n = 6/group) as follows: normally-fed controls (CON), fasted for 24 h (24F), fasted for 48 h (48F), and fed normally for 2 d after fasting for 48 h (FAF). Biochemical measures were analyzed by gas chromatography (GC) and high performance liquid chromatography (HPLC). Our results demonstrate that hypothalamic saturated fatty acid (C16:0, C18:0) levels were lower, and unsaturated fatty acid (C22:6 n-3, C22:4 n-6, C20:4 n-6) levels were higher, in the 48F and FAF groups than in CON ( P < 0 . 01 ). In addition, hypothalamic monounsaturated fatty acid (MUFA) levels were lower, and polyunsaturated fatty acids (PUFA) levels were higher, in 24F than in CON. Total hypothalamic lipids in both 24F and 48F were higher than CON, and cholesterol was elevated in the hypothalami of all experimental groups as compared to CON. Serum malondialdehyde was higher in fasted and FAF groups; 24F and 48F also had higher serum cholesterol levels, than CON ( P < 0 . 01 , P < 0 . 001 ). Serum α-tocoferol, retinol and vitamin C values were lower in 48F than CON ( P < 0 . 05 ). In conclusion, we demonstrate that feeding state can significantly alter brain fatty acid and lipid derivative levels, and serum concentrations of cholesterol and vitamins. These changes may consequently influence lipid peroxidation, fatty acid synthase or desaturase system in hypothalamic fields.

Affiliations: 1: 1Ahi Evran University, Art And Science Faculty, Department of Biology, 40100 Kirsehir, Turkey; 2: 2The University of Texas at El Paso, Department of Biological Sciences and Border Biomedical Research Center, El Paso, TX 79968, USA; 3: 3Firat University, Faculty of Science, Department of Biology, 23169- Elazig, Turkey

10.1163/157075611X616897
/content/journals/10.1163/157075611x616897
dcterms_title,pub_keyword,dcterms_description,pub_author
6
3
Loading
Loading

Full text loading...

/content/journals/10.1163/157075611x616897
Loading

Data & Media loading...

1. Agale S. , Kulkarni A. , Ranjekar P. , Joshi S. ( 2010) "Maternal caloric restriction spares feral brain polyunsaturated fatty acids in Wistar rats". Brain & Dev., Vol 32, 123- 129. http://dx.doi.org/10.1016/j.braindev.2008.12.001
2. Awad S. , Constantin-Teodosiu D. , Macdonald I.A. , Lobo D.N. ( 2009) "Short-term starvation and mitochondrial dysfunction – a possible mechanism leading to postoperative insulin resistance". Clin. Nutr., Vol 28, 497- 509. http://dx.doi.org/10.1016/j.clnu.2009.04.014
3. Awasthi Y.C. , Yang Y. , Tiwari N.K. , Patrick B. , Sharma A. , Li J. , Awasthi S. ( 2004) "Regulation of 4-hydroxynonenal-mediated signaling by glutathione S-transferases". Free Radical Bio. Med., Vol 37( 5), 607- 619. http://dx.doi.org/10.1016/j.freeradbiomed.2004.05.033
4. Barazzoni R. , Bosutti A. , Stebel M. , Cattin M.R. , Roder E. , Visintin L. , Cattin L. , Biolo G. , Zanetti M. , Guarnieri G. ( 2005) "Ghrelin regulates mitochondrial-lipid metabolism gene expression and tissue fat distribution in liver and skeletal muscle". Am. J. Physiol. Endoc. M., Vol 288, E228- E235.
5. Bertile F. , Oudart H. , Criscuolo F. , Maho Y.L. , Raclot T. ( 2003) "Hypothalamic gene expression in long-term fasted rats: relationship with body fat". Biochem. Bioph. Res. Co., Vol 303, 1106- 1113. http://dx.doi.org/10.1016/S0006-291X(03)00481-9
6. Borcea V. , Nourooz-Zadeh J. , Wolf S.P. , Klevesath M. , Hofmann M. , Urich H. ( 1999) "Alpha-lipoic acid decreases oxidative stres even in diabetic patients with poor glycemic control and albuminuria". Free Radical Bio. Med., Vol 26( 11-12), 1495- 1500. http://dx.doi.org/10.1016/S0891-5849(99)00011-8
7. Chakravarthy M.V. , Zhu Y. , Lopez M. , Yin L. , Wozniak D.F. , Coleman T. , Hu Z. , Wolfgang M. , Vidal-Puig A. , Lane M.D. , Semenkovich C.F. ( 2007) "Brain fatty acid synthase activates PPARα to maintain energy homeostasis". J. Clin. Invest., Vol 117, 2539- 2552. http://dx.doi.org/10.1172/JCI31183
8. Chibaa T. , Komatsua T. , Nakayamaa M. , Adachia T. , Tamashiroa Y. , Hayashia H. , Yamazaa H. , Higamib Y. , Shimokawa I. ( 2009) "Similar metabolic responses to calorie restriction in lean and obese Zucker rats". Mol. Cell Endocrinol., Vol 309, 17- 25. http://dx.doi.org/10.1016/j.mce.2009.05.001
9. Chow C.K. ( 1991) "Vitamin E and oxidative stress". Free Radical Biol. Med., Vol 11( 2), 215- 232. http://dx.doi.org/10.1016/0891-5849(91)90174-2
10. Christie W.W. ( 1992) Gas Chromatography and Lipids. The Oil Pres, Glaskow, 302 pp.
11. Currie P.J. , Coscina D.V. ( 1995) "Dissociated feeding and hypothermic effects of neuropeptide Y in the paraventricular and perifornical hypothalamus". Peptides, Vol 16, 599- 604. http://dx.doi.org/10.1016/0196-9781(95)00020-K
12. Del Rio D. , Stewart A.J. , Pellegrini N. ( 2005) "A review of recent studies on malonaldehyde as toxic molecule and biological marker of oxidative stress". Nutr. Metab. Cardiovas., Vol 15( 4), 316- 328. http://dx.doi.org/10.1016/j.numecd.2005.05.003
13. Diorio D. , Viau V. , Meaney M.J. ( 1993) "The role of the medial prefrontal cortex (cingulate gyrus) in the regulation of hypothalamic-pituitaryadrenal responses to stress". J. Neurosci., Vol 13, 3839- 3847.
14. Dowell P. , Hu Z. , Lane M.D. ( 2005) "Monitoring energy balance: metabolites of fatty acid synthesis as hypothalamic sensors". Annu. Rev. Biochem., Vol 74, 515- 534. http://dx.doi.org/10.1146/annurev.biochem.73.011303.074027
15. Druce M. , Bloom S.R. ( 2006) "The regulation of appetite". Arch. Dis. Child., Vol 91, 183- 187. http://dx.doi.org/10.1136/adc.2005.073759
16. Fernandez M.L. , Vega S. , Ayala M.T. , Shen H. , Conde K. , Vergara-Jimenez M. , Robbins A. ( 1997) "Vitamin C level and dietary fat saturation alter hepatic cholesterol homeostasis and plasma LDL metabolism in guinea pigs". J. Nutr. Biochem., Vol 8( 7), 414- 424. http://dx.doi.org/10.1016/S0955-2863(97)00050-8
17. Frenkel A.L. , Canetti L. , Halpern Z. ( 2004) "Effects of the nuritional lipids on diabetic manifestation and D6 desaturase mRNA in Streptozotocin treated mice". Nutr. Res., Vol 24, 3003- 3012. http://dx.doi.org/10.1016/j.nutres.2003.11.006
18. Froiland E. , Murashita K. , Jorgensen E.H. , Kurokawa T. ( 2010) "Leptin and ghrelin in anadromous Arctic charr: cloning and change in expressions during a seasonal feeding cycle". Gen. Comp. Endocr., Vol 165, 136- 143. http://dx.doi.org/10.1016/j.ygcen.2009.06.010
19. Gönül B. , Kaplan B. ( 1999) "Effects of vitamin C supplementation on plasma antioxidant status in unfed periods". Gen. Pharmacol., Vol 32( 2), 195- 199. http://dx.doi.org/10.1016/S0306-3623(98)00195-5
20. Hara A. , Radin N.S. ( 1978) "Lipid extraction of tissues with a low-toxicity solvent". Anal. Biochem., Vol 90, 420- 426. http://dx.doi.org/10.1016/0003-2697(78)90046-5
21. Herman J.P. , Figueiredo H. , Mueller N.K. , Ulrich-Lai Y. , Ostrander M.M. , Choi D.C. , Cullinan W.E. ( 2003) "Central mechanisms of stress integration: hierarchical circuitry controlling hypothalamo-pituitary-adrenocortical responsiveness". Front. Neuroendocrinol., Vol 24, 151- 180. http://dx.doi.org/10.1016/j.yfrne.2003.07.001
22. Hu Z. , Cha S.H. , Chohnan S. , Lane M.D. ( 2003) "Hypothalamic malonyl-CoA as amediator of feeding behavior". P. Natl. Acad. Sci. USA, Vol 100, 12624- 12629. http://dx.doi.org/10.1073/pnas.1834402100
23. Hu Z. , Cha S.H. , Van Haasteren G. , Wang J. , Lane M.D. ( 2005) "Effect of centrally administered C75, a fatty acid synthase inhibitor, on ghrelin secretion and its downstream effects". P. Natl. Acad. Sci. USA, Vol 102, 3972- 3977. http://dx.doi.org/10.1073/pnas.0500619102
24. Kahn B.B. , Alquier T. , Carling D. , Hardie D.G. ( 2005) "AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism". Cell Metab., Vol 1, 15- 25. http://dx.doi.org/10.1016/j.cmet.2004.12.003
25. Kara H. , Karatas F. , Canatan H. , Servi K. ( 2005) "Effects of exogenous metallothionein on acute cadmium toxicity in rats". Biol. Trace Elem. Res., Vol 104, 223- 232. http://dx.doi.org/10.1385/BTER:104:3:223
26. Kim E.K. , Miller I. , Landree L.E. , Borisy-Rudin F.F. , Brown P. , Tihan T. ( 2002) "Expression of FAS within hypothalamic neurons: a model for decreased food intake after C75 treatment". Am. J. Physiol. Endoc. M., Vol 283, E867- E879.
27. Lam T.K. , Schwartz G.J. , Rossetti L. ( 2005) "Hypothalamic sensing of fatty acids". Nat. Neurosci., Vol 8, 579- 584. http://dx.doi.org/10.1038/nn1456
28. Lane M.D. , Hu Z. , Cha S.H. , Wolfgang M. , Sidhaye A. ( 2005) "Role of malonyl-CoA in the hypothalamic control of food intake and energy expenditure". Biochem. Soc. T., Vol 33, 1063- 1067. http://dx.doi.org/10.1042/BST20051063
29. Li R.Y. , Zhang Q.H. , Liu Z. , Qiao J. , Zhao S.X. , Shao L. , Xiao H.S. , Chen J.L. , Chen M.D. , Song H.D. ( 2006) "Effect of short-term and long-term fasting on transcriptional regulation of metabolic genes in rat tissues". Biochem. Bioph. Res. Co., Vol 344, 562- 570. http://dx.doi.org/10.1016/j.bbrc.2006.03.155
30. Loftus T.M. , Jaworsky D.E. , Frehywot G.L. , Townsend C.A. , Ronnett G.V. , Lane M.D. , Kuhajda F.P. ( 2000) "Reduced food intake and body weight in mice treated with fatty acid synthase inhibitors". Science, Vol 288, 2379- 2381. http://dx.doi.org/10.1126/science.288.5475.2379
31. López M. , Tovar S. , Vázquez M.J. , Nogueiras R. , Seňarís R. , Diéguez C. ( 2005) "Sensing the fat: fatty acid metabolism in the hypothalamus and the melanocortin system". Peptides, Vol 26, 1753- 1758. http://dx.doi.org/10.1016/j.peptides.2004.11.025
32. López M. , Lelliott C.J. , Tovar S. , Kimber W. , Gallego R. , Virtue S. , Blount M. , Vázquez M.J. , Finer N. , Powles T. ( 2006) "Tamoxifen-induced anorexia is associated with fatty acid synthase inhibition in the ventromedial nucleus of the hypothalamus and accumulation of malonyl-CoA". Diabetes, Vol 55, 1327- 1336. http://dx.doi.org/10.2337/db05-1356
33. López M. , Lage R. , Saha A.K. , Perez-Tilve D. , Vázquez M.J. , Varela L. , Sangiao-Alvarellos S. , Tovar S. , Raghay K. , Rodriguez-Cuenca S. , Deoliveira R.M. , Castaneda T. , Data T. , Dong J.Z. , Culler M. , Sleeman M.W. , Alvarez C.V. , Gallego R. , Lelliott C.J. , Carling D. , Tschop M.H. , Diéguez C. , Vidal-Puig A. ( 2008) "Hypothalamic fatty acid metabolism mediates the orexigenic action of ghrelin". Cell Metab., Vol 7, 389- 399. http://dx.doi.org/10.1016/j.cmet.2008.03.006
34. Meister B. ( 2007) "Neurotransmitters in key neurons of the hypothalamus that regulate feeding behaviour and body weight". Physiol. Behav., Vol 92, 263- 271. http://dx.doi.org/10.1016/j.physbeh.2007.05.021
35. Miyazaki M. , Kim Y.C. , Ntambi J.M. ( 2001) "A lipogenic diet in mice with a disruption of the stearoyl-CoA desaturase 1 gene reveals a stringent requirement of endogenous monounsaturated fatty acids for triglyceride synthesis". J. Lipid Res., Vol 42, 1018- 1024.
36. Nogueiras R. , Wilson H. , Rohner-Jeanrenaud F. , Tschöp M.H. ( 2008) "Central nervous system regulation of adipocyte function". Regul. Peptides., Vol 149, 26- 31. http://dx.doi.org/10.1016/j.regpep.2007.09.034
37. Padayatty S.J. , Katz A. , Wang Y. , Eck P. , Kwon O. , Lee J.H. , Chen S. , Corpe C. , et al   ( 2003) "Vitamin C as an antioxidant: evaluation of its role in disease prevention". J. Am. Coll. of Nutr., Vol 22( 1), 18- 35. http://dx.doi.org/10.1080/07315724.2003.10719272
38. Paton C.M. , Ntambi J.M. ( 2009) "Biochemical and physiological function of stearoyl-CoA desaturase". Am. J. Physiol. Endoc. M., Vol 297, 28- 37. http://dx.doi.org/10.1152/ajpcell.00476.2008
39. Pilegaard H. , Saltin B. , Neufer P.D. ( 2003) "Effect of short-term fasting and refeeding on transcriptional regulation of metabolic genes in human skeletal muscle". Diabetes, Vol 52, 657- 662. http://dx.doi.org/10.2337/diabetes.52.3.657
40. Poisson J.P.G. , Cunnane S.C. ( 1991) "Long-chain fatty acid metabolism in fasting and diabetes: relation between altered desaturase activity and fatty acid composition". J. Nutr. Biochem., Vol 2( 2), 60- 70. http://dx.doi.org/10.1016/0955-2863(91)90030-9
41. Qu P. , Tritschler H.J. , Wolff S.P. ( 1995) "Thioctic (lipoic) acid: a therapeutic metal-chelating antioxidant". Biochem. Pharmacol., Vol 50, 123- 126. http://dx.doi.org/10.1016/0006-2952(95)00116-H
42. Ramosa E.J.B. , Romanova I.V. , Suzukia S. , Chenb C. , Ugrumovc M.V. , Satoa T. , Goncalvesa C.G. , Meguid M.M. ( 2005) "Effects of omega-3 fatty acids on orexigenic and anorexigenic modulators at the onset of anorexia". Brain Res., Vol 1046, 157- 164. http://dx.doi.org/10.1016/j.brainres.2005.03.052
43. Reyes T.M. , Lewis K. , Perin M.H. , Kunitake K.S. , Vaughan J. , Arias C.A. , Hogenesch J.B. , Gulyas J. , Rivier J. , Vale W.W. , Sawchenko P.E. ( 2001) "Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors". P. Natl. Acad. Sci. USA, Vol 98, 2843- 2848. http://dx.doi.org/10.1073/pnas.051626398
44. Robin J.P. , Frain M. , Sardet C. , Groscolas R. , Le Maho Y. ( 1998) "Protein and lipid utilization during long-term fasting in emperor penguins". Am. J. Physiol. Reg. I., Vol 254, R61- R68.
45. Ronnett G.V. , Kima E.K. , Landreea L.E. , Tu Y. ( 2005) "Fatty acid metabolism as a target for obesity treatment". Physiol. Behav., Vol 85, 25- 35. http://dx.doi.org/10.1016/j.physbeh.2005.04.014
46. Sainsburya A. , Zhang L. ( 2010) "Role of the arcuate nucleus of the hypothalamus in regulation of body weight during energy deficit". Mol. Cell Endocrinol., Vol 316, 109- 119. http://dx.doi.org/10.1016/j.mce.2009.09.025
47. Sarsilmaz M. , Songur A. , Ozyurt H. , Kus I. , Ozen O.A. , Ozyurt B. , Sogut S. , Akyol O. ( 2003) "Potential role of dietary omega-3 essential fatty acids on some oxidant/antioxidant parameters in rats’ corpus striatum". Prostag. Leukotr. Ess., Vol 69, 253- 259. http://dx.doi.org/10.1016/S0952-3278(03)00107-8
48. Schwartz M.W. , Woods S.C. , Porte D. Jr. , Seeley R.J. , Baskin D.G. ( 2000) "Central nervous system control of food intake". Nature, Vol 404, 661- 671.
49. Shimokawa T. , Kumar M.V. , Lane M.D. ( 2002) "Effect of a fatty acid synthase inhibitor on food intake and expression of hypothalamic neuropeptides". P. Natl. Acad. Sci. USA, Vol 99, 66- 71. http://dx.doi.org/10.1073/pnas.012606199
50. Sul H.S. , Wang D. ( 1998) "Nutritional and hormonal regulation of enzymes in fat synthesis: studies of fatty acid synthase and mitochondrial glycerol-3-phosphate acyltransferase gene transcription". Annu. Rev. Nutr., Vol 18, 331- 351. http://dx.doi.org/10.1146/annurev.nutr.18.1.331
51. Swierczynski J. , Goyke E. , Korczynska J. , Jankowski Z. ( 2008) "Acetyl-CoA carboxylase and fatty acid synthase activities in human hypothalamus". Neurosci. Lett., Vol 444, 209- 211. http://dx.doi.org/10.1016/j.neulet.2008.08.046
52. Turpeinen A.M. , Basu S. , Mutanen M.A. ( 1998) "High linoleic acid diet increases oxidative stress in vivo and affects nitric oxide metabolism in humans". Prostag. Leukotr. Ess., Vol 59, 229- 233. http://dx.doi.org/10.1016/S0952-3278(98)90067-9
53. Vaughan J. , Donaldson C. , Bittencourt J. , Perin M.H. , Lewis K. , Sutton S. , Chan R. , Turnbull A.V. , Lovejoy D. , Rivier C. , Rivier J. , Sawchenko P.E. , Vale W. ( 1995) "Urocortin, a mammalian neuropeptide related to fish urotensin I and to corticotropin-releasing factor". Nature, Vol 378, 287- 292. http://dx.doi.org/10.1038/378287a0
54. Wakil S.J. ( 1989) "Fatty acid synthase, a proficient multifunctional enzyme". Biochemistry, Vol 28, 4523- 4530. http://dx.doi.org/10.1021/bi00437a001
55. Wolf G. ( 1996) "Nutritional and hormonal regulation of fatty acid synthase". Nutr. Rev., Vol 54, 122- 123. http://dx.doi.org/10.1111/j.1753-4887.1996.tb03886.x
56. Wolfgang M.J. , Kurama T. , Dai Y. , Suwa A. , Asaumi M. , Matsumoto S. , Cha S.H. , Shimokawa T. , Lane M.D. ( 2006) "The brain-specific carnitine palmitoyltransferase-1c regulates energy homeostasis". P. Natl. Acad. Sci. USA, Vol 103, 7282- 7287. http://dx.doi.org/10.1073/pnas.0602205103
57. Yen Y.H. , Lee Y.T. , Hsieh H.S. , Hwang D.F. ( 2008) "Effect of taurine on toxicity of vitamin A in rats". Food Chem., Vol 106, 260- 268. http://dx.doi.org/10.1016/j.foodchem.2007.05.084
58. Zaidi S.M.K.R. , Banu N. ( 2004) "Antioxidant potential of vitamins A, E and C in modulating oxidative stress in rat brain". Clin. Chim. Acta, Vol 340, 229- 233. http://dx.doi.org/10.1016/j.cccn.2003.11.003
59. Zolfaghari R. , Ross C.A. ( 2003) "Recent advances in molecular cloning of fatty acid desaturase genes and the regulation of their expression by dietary vitamin A and retinoic acid". Prostag. Leukotr. Ess., Vol 68, 171- 179. http://dx.doi.org/10.1016/S0952-3278(02)00267-3
http://brill.metastore.ingenta.com/content/journals/10.1163/157075611x616897
Loading

Article metrics loading...

/content/journals/10.1163/157075611x616897
2012-01-01
2016-12-08

Sign-in

Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
     
    Animal Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation