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Effects of Tripterygium wilfordii multiglycosides on sex hormones and receptors in mandarin vole Leydig cells

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For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Netherlands Journal of Zoology (Vol 18-52).

Multiglycosides of Tripterygium wilfordii (GTW) have been found to exhibit reversible antifertility effects through complex mechanisms. The positive expression of sex hormones and receptors in Leydig cells were investigated following GTW treatment to determine the impact of GTW on male mandarin vole (Microtus mandarinus) fertility. Compared to the control group, body weight, testis and epididymis weight were not affected by GTW, but the number of sperm sharply declined. There were significant decreases in the thickness of seminifeous epithelium and epididymal epithelium, and the diameter of seminiferous tubules. The expression of estrogen receptor alpha (ERα) in Leydig cells significantly decreased and estradiol (E) expression significantly increased. Testosterone (T) and androgen receptor (AR) expression were not affected. These results show that GTW may impair fertility in male mandarin voles via interference with spermatogenesis. This may be associated with an alteration in estrogen activation rather than suppression of T and AR in Leydig cells and suggests the estrogen system of Leydig cells is of major importance for the GTW’s antifertility effects.

Affiliations: 1: 1College of Life Sciences, Shaanxi Normal University, Xi’an 710062, China

10.1163/157075611X618174
/content/journals/10.1163/157075611x618174
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Effects of multiglycosides on sex hormones and receptors in mandarin vole Leydig cells
Animal Biology 62, 2, 217 (2012); https://doi.org/10.1163/157075611X618174
/content/journals/10.1163/157075611x618174
/content/journals/10.1163/157075611x618174
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1. Bai J.P. , Shi Y.L. ( 2002) "Inhibition of Ca 2+channels in mouse spermatogenic cells by male antifertility compounds from Tripterygium wilfordiiHook.f." Contraception, Vol 65, 441- 445. [Crossref]
2. Bai J.P. , Shi Y.L. , Fang X. , Shi Q.X. ( 2003) "Effects of demethylzeylasteral and celastrol on spermatogenic cell Ca 2+channels and progesterone-induced sperm acrosome reaction". Eur. J. Pharmacol., Vol 464, 9- 15. [Crossref]
3. Brinker A.M. , Ma J. , Lipsky P.E. , Raskin I. ( 2007) "Medicinal chemistry and pharmacology of genus Tripterygium (Celastraceae)". Phytochemistry, Vol 68, 732- 766. [Crossref]
4. Carballada R. , Saling P.M. ( 1997) "Regulation of mouse epididymal epithelium in vitro by androgens, temperature and fibroblasts". J. Reprod. Fertil., Vol 110, 171- 181. [Crossref]
5. Hikim A.P. , Lue Y.H. , Wang C. , Reutrakul V. , Sangsuwan R. , Swerdloff R.S. ( 2000) "Posttesticular antifertility action of triptolide in the male rat: evidence for severe impairment of cauda epididymal sperm ultrastructure". J. Androl., Vol 21, 431- 437.
6. Huo X.F. , Wang D. , Guo Y.W. , Shi D.Z. ( 2008) "Effect of preparations from Tripterygium wilfordiionreproduction of Mongolian gerbils ( Meriones unguiculatus)". Acta Theriol. Sin., Vol 28, 305- 310.
7. Ikegawa S. , Hata J. , Nakatomi K. , Asaga H. , Kaji M. , Sugawara S. , Uno H. , Izawa Y. ( 1995) "Collaborative work to determine the optimal administration period and parameters to detect drug effects on male rat fertility". J. Toxicol. Sci., Vol 20, 251- 263. [Crossref]
8. Iwase T. , Sano F. , Murakami T. , Inazawa K. ( 1995) "Male reproductive toxicity of ethinylestradiol associated with 4 weeks daily dosing prior to mating in rats". J. Toxicol. Sci., Vol 20, 265- 279. [Crossref]
9. Kaneto M. , Kanamori S. , Hishikawa A. , Kishi K. ( 1999) "Epididymal sperm motion as a parameter of male reproductive toxicity: sperm motion, fertility, and histopathology in ethinylestradiol-treated rats". Reprod. Toxicol., Vol 13, 279- 289. [Crossref]
10. Kotula M. , Tuz R. , Frczek B. , Wojtusiak A. , Bilińska B. ( 2000) "Immunolocalization of androgen receptors in testicular cells of prepubertal and pubertal pigs". Folia Histochem. Cytobiol., Vol 38, 157- 162.
11. Lan Z.J. , Gu Z.P. , Lu R.F. , Zhuang L.Z. ( 1992) "Effects of multiglycosides of Tripterygium wilfordii(GTW) on rat fertility and Leydig and Sertoli cells". Contraception, Vol 45, 249- 261. [Crossref]
12. Li J.M. , Zhang M. , Guo Y.W. , Hai S.Z. , Wu X.P. , Ji L.L. , Shi D.Z. ( 2009) "The sterility effect of tripterygium glucosides ( Tripterygium wilfordii) on male Brandt’s voles". Acta Theriol. Sin., Vol 29, 69- 74.
13. Li X.Y. ( 1993) "Anti-inflammatory and immunosuppressive components of Tripterygium wilfordiiHook. f." Int. J. Immunother, Vol 9, 181- 187.
14. Lue Y. , Sinha Hikim A.P. , Wang C. , Leung A. , Baravarian S. , Reutrakul V. , Sangsawan R. , Chaichana S. , Swerdloff R.S. ( 1998) "Triptolide: a potential male contraceptive". J. Androl., Vol 19, 479- 486.
15. Mruk D.D. , Cheng C.Y. ( 2004) "Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis". Endocr. Rev., Vol 25, 747- 806. [Crossref]
16. O’Donnell L. , Meachem S.J. , Stanton P.G. , McLachlan R.I. ( 2006) "Endocrine regulation of spermatogenesis". In: Neill J. D. (Ed.) Knobil and Neill’s Physiology of Reproduction, pp. 1017- 1069. Elsevier Academic Press. [Crossref]
17. Papadopoulos V. , Carreau S. , Szerman-Joly E. , Drosdowsky M.A. , Dehennin L. , Scholler R. ( 1986) "Rat testis 17-beta estradiol: identification by gas chromatogarphy-mass spectrometry and age-related cellular distribution". J. Steroid. Biochem., Vol 24, 1211- 1216. [Crossref]
18. Payne A.H. , Kelch R.P. , Musich S.S. , Halpern M.E. ( 1976) "Intratesticular site of aromatization in the human". J. Clin. Endocrinol. Metab., Vol 114, 1081- 1087. [Crossref]
19. Qian S.Z. ( 1987). " Tripterygium wilfordii, a Chinese herb effective in male fertility regulation". Contraception, Vol 36, 335- 345. [Crossref]
20. Qian S.Z. , Zhong C.Q. , Xu Y. ( 1986) "Effect of Tripterygium wilfordiion the fertility in rats". Contraception, Vol 33, 105- 110. [Crossref]
21. Qian S.Z. , Ye X. , Wei Z.J. ( 1995) "Recent progress in research on Tripterygium: a male antifertility plant". Contraception, Vol 51, 121- 129. [Crossref]
22. Rao M.V. , Chinoy N.J. ( 1983) "Effect of estradiol benzoate on reproductive organs and fertility in the male rat". Eur. J. Obstet. Gynecol. Reprod. Biol., Vol 15, 189- 198. [Crossref]
23. Ren Y.P. , Sun L. , Jiang W. , Hu C.P. ( 2005) "Effects of glycosides of Tripterygium wilfordii, methyltestosterone and zhuanggushenjin capsule on nitric oxide synthase in rat testes". Natl. J. Androl., Vol 11, 343- 345, 349.
24. Saez J.M. ( 1994) "Leydig cells: endocrine, paracrine and autocrine regulation". Endocr. Rev., Vol 15, 574- 626. [Crossref]
25. Seegers J.C. , Nieuwoudt L.B. , Joubert W.S. ( 1991) "Morphological effects of the catechol estrogens on rat epididymal epithelia". J. Steroid Biochem. Mol. Bio., Vol 138, 717- 720. [Crossref]
26. Sharma R.S. , Rajalakshmi M. , Sharma R.S. , Jeyaraj D.A. ( 2001) "Current status of fertility control methods in India". J. Biosci., Vol 26, 391- 405. [Crossref]
27. Singh J. , Adams G.P. ( 1998). "Immunohistochemical distribution of follistatin in dominant and subordinate follicles and the corpus luteum of cattle". Biol. Reprod., Vol 59, 561- 570. [Crossref]
28. Steinberger E. , Duckett G.E. ( 1965) "Effect of estrogen or testosterone on initiation and maintenance of spermatogenesis in the rat". Endocrinology, Vol 76, 1184- 1189. [Crossref]
29. Strader L.F. , Linder R.E. , Perreault S.D. ( 1996) "Comparison of rat epididymal sperm counts by IVOS HTM-IDENT and hemacytometer". Reprod. Toxicol., Vol 10, 529- 533. [Crossref]
30. Tai F.D. , Wang T.Z. ( 2001) "Social organization of mandarin voles in burrow system". Acta Theriol. Sin., Vol 21, 50- 56.
31. Tai F.D. , Wang T.Z. , Zhao Y.J. ( 2001) "Mating system of mandarin vole ( Microtus mandarinus)". Acta Zool. Sin., Vol 47, 260- 267.
32. Tao X.L. , Davis L.S. , Lipsky P.E. ( 1991) "Effect of an extract of Chinese herbal remedy Tripterygium wilfordiiHook.f. on human immune responses". Arthritis Rheum, Vol 34, 1274- 1281. [Crossref]
33. Tao X. , Lipsky P.E. ( 2000) "The Chinese anti-inflammatory and immunosuppressive herbal remedy Tripterygium wilfordiiHook. f." Rheum. Dis. Clin. N. Amer., Vol 26, 29- 50. [Crossref]
34. Verjans H.L. , de Jong F.H. , Cooke B.A. , van der Molen H.J. , Eik-Nes K.B. ( 1974) "Effects of estradiol benzoate on pituitary and testis function in the normal adult male rats". Acta Endocrinol., Vol 77, 636- 642.
35. Vigueras-Villaseñor R.M. , Moreno-Mendoza N.A. , Reyes-Torres G. , Molina-Ortiz D. , León M.C. , Rojas-Castañeda J.C. ( 2006) "The effect of estrogen on testicular gonocyte maturation". Reprod. Toxicol., Vol 22, 513- 520. [Crossref]
36. Wang H.C. , Tai F.D. , Lian Y. ( 2005) "Age-specific changes in estrogen receptors alpha in testis and epididymis of mandarin voles ( Microtus mandarinus)". Zool. Res., Vol 26, 435- 441.
37. Wang J.L. , Wang H.C. , Tai F.D. ( 2009) "Age dependent expression of androgen receptor in testes and epididymis of mandarin voles ( Microtus mandarinus)". Zool. Res., Vol 30, 53- 58. [Crossref]
38. Wang L. , Ye W. , Hui L. , Liu X. , Guo Y. ( 2000) "Male contraception of triptolide and its function mechanism". Acta Acad. Med. Sin., Vol 22, 223- 226.
39. Wu F.C. ( 1988) "Male contraception: current status and future prospects". Clin. Endocrinol. (Oxf.), Vol 29, 443- 465. [Crossref]
40. Wu T. , Sha Y. ( 1996) "Tripchlorolide inhibition of Ca 2+influx in human ejaculated sperm, but not affecting contraction reaction of aorta and vas deferens in rats". Chin. Pharm. Bull., Vol 5, 441- 444.
41. Ye W.S. , Deng Y.C. , Huang Y.Q. , Xue S.P. ( 1991) "Genetic effects of tripchlorolide on germ cells in rats". Chin. J. Androl., Vol 5, 130- 132.
42. Zhang C.M. , Wang R.H. ( 2002) "Advance in the research of sterilants against rodents". J. For. Res., Vol 13, 77- 81. [Crossref]
43. Zhou X. , Kudo A. , Kawakami H. , Hirano H. ( 1996) "Immunohistochemical localization of androgen receptor in mouse testicular germ cells during fetal and postnatal development". Anat. Rec., Vol 245, 509- 518. [Crossref]
44. Zhu L.J. , Hardy M.P. , Inigo I.V. , Huhtaniemi I. , Bardin C.W. , Moo-Young A.J. ( 2000) "Effects of androgen on androgen receptor expression in rat testicular and epididymal cells: a quantitative immunohistochemical study". Biol. Reprod., Vol 63, 368- 376. [Crossref]
45. Zirkin B.R. ( 1998) "Spermatogenesis: its regulation by testosterone and FSH". Semin. Cell. De. Biol., Vol 9, 417- 421. [Crossref]
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/content/journals/10.1163/157075611x618174
2012-01-01
2017-06-27
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