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Sexually dimorphic gene expression patterns during gonadal differentiation in olive flounder, Paralichthys olivaceus

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image of Animal Biology
For more content, see Archives Néerlandaises de Zoologie (Vol 1-17) and Netherlands Journal of Zoology (Vol 18-52).

The present study aims to elucidate the different expression patterns and possible roles of Doublesex and Mab-3-related transcription factor 1 ( dmrt1), dmrt4, SRY-related transcription factor 9 ( sox9) and cytochrome P450 aromatase 19a ( cyp19a) during gonadal differentiation in olive flounder, Paralichthys olivaceus. We first analyzed the gene expression patterns in tissues using RT-PCR, which indicated dmrt1, sox9and cyp19awere sex-related genes with sexual dimorphic expression. The quantitative expression changes of these three genes together with dmrt4during gonadal differentiation were further examined using real-time RT-PCR. The results showed that dmrt1was scarcely expressed in the primitive gonad and during following periods of gonadal differentiation. Its expression increased rapidly in the differentiating testis. Dmrt4was strongly expressed in primitive gonads and much less expressed during following periods of gonadal differentiation. Its expression became strong in differentiating testes. While sox9was highly expressed in the primitive gonad, it was expressed with fluctuations during following periods of gonadal differentiation. Cyp19astarted expressing in primitive gonads, and its expression quantity fluctuated during latter periods of gonadal differentiation, but was strongly expressed in the early stage of differentiating ovaries. Results of in situhybridization showed that dmrt4and sox9transcripts were both mainly localized in spermatocytes and our results suggested these four sex-related genes might be involved in gonadal differentiation through their synergistic effects in flounder.

Affiliations: 1: 1Key Laboratory of Experimental Marine Biology, National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People’s Republic of China

10.1163/15707563-00002470
/content/journals/10.1163/15707563-00002470
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/content/journals/10.1163/15707563-00002470
2015-11-23
2017-12-14

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