Cookies Policy

This site uses cookies. By continuing to browse the site you are agreeing to our use of cookies.

I accept this policy

Find out more here

Gender-specific differences in gene expression profiles in gynogenetic Pengze crucian carp

No metrics data to plot.
The attempt to load metrics for this article has failed.
The attempt to plot a graph for these metrics has failed.
The full text of this article is not currently available.

Brill’s MyBook program is exclusively available on BrillOnline Books and Journals. Students and scholars affiliated with an institution that has purchased a Brill E-Book on the BrillOnline platform automatically have access to the MyBook option for the title(s) acquired by the Library. Brill MyBook is a print-on-demand paperback copy which is sold at a favorably uniform low price.

Access this article

+ Tax (if applicable)
Add to Favorites
You must be logged in to use this functionality

image of Animal Biology

Gynogenesis is a form of asexual reproduction that is used to obtain all-female fish stocks. In this study, we were interested in studying gender-specific differences in gene expression profiles in gynogenetic teleosts, using a carp species. The four-month old gynogenetic Pengze crucian carp F1 (Carassius auratus var. pengzensis, Pcc) showed a high ratio of males under laboratory culture condition. The present study aimed to investigate the differences between males and females. The gonadosomatic index of the females was significantly higher than that of the males. Moreover, the hepatosomatic index of the females was significantly lower than that of the males. Vitellogenin B mRNA was abnormally highly expressed in male hepatopancreas and testes compared to females. Similarly, zona pellucida 2 expressed at a significantly high level in the testes. For the sex related genes, dosage-sensitive sex reversal, adrenal hypoplasia congenital critical region on the X-chromosome gene 1, doublesex and mab-3 related transcription factor 1a, nuclear receptor subfamily 5, group A, member 1b and SRY-box containing gene 9a had significantly higher expression levels in the males than in the females, whereas there was no difference in expression of anti-Müllerian hormone, cytochrome P450 family 19 subfamily A member 1A and forkhead box L2 transcripts between the two genders. The females showed higher levels of estrogen but no significant difference in testosterone compared to the males. The data suggest remarkable differences between the two genders of the Pengze crucian carp.

Affiliations: 1: 1Freshwater Fisheries Research Center, Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries, Chinese Academy of Fishery Sciences; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Changjiang River, Ministry of Agriculture; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2: 3Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, Hubei 430223, China; 3: 2College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China

Loading data from figshare Loading data from figshare

Full text loading...


Data & Media loading...

1. Chourrout D. , Quillet E. (1982) "Induced gynogenesis in the rainbow trout: sex and survival of progenies production of all-triploid populations". Theor. Appl. Genet., Vol 63, 201-205.
2. Felip A. , Piferrer F. , Carrillo M. , Zanuy S. (2001b) "Comparison of the gonadal development and plasma levels of sex steroid hormones in diploid and triploid sea bass, Dicentrarchus labrax L." J. Exp. Zool., Vol 290, 384-395.
3. Felip A. , Zanuy S. , Carrillo M. , Piferrer F. (2001a) "Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species". Genetica, Vol 111, 175-195.
4. Gomez-Requeni P. , Conceicao L.E.C. , Olderbakk J.A.E. , Ronnestad I. (2010) "A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development". Fish Physiol. Biochem., Vol 36, 1199-1215.
5. Heidari B. , Roozati S.A. , Yavari L. (2010) "Changes in plasma levels of steroid hormones during oocyte development of Caspian Kutum (Rutilus frisii kutum, Kamensky, 1901)". Anim. Reprod., Vol 7, 373-381.
6. Ito L.S. , Cornejo A.M. , Yamashita M. , Strüssmann C.A. (2008) "Thermal threshold and histological process of heat-induced sterility in adult pejerrey (Odontesthes bonariensis): a comparative analysis of laboratory and wild specimens". Physiol. Biochem. Zool., Vol 81, 775-784.
7. Jobling S. , Nolan M. , Tyler C.R. , Brighty G. , Sumpter J.P. (1998) "Widespread sexual disruption in wild fish". Environ. Sci. Technol., Vol 32, 2498-2506.
8. Kobayashi T. , Kajiura-Kobayashi H. , Guan G. , Nagahama Y. (2008) "Sexual dimorphic expression of DMRT1 and Sox9a during gonadal differentiation and hormone-induced sexreversal in the teleost fish Nile tilapia (Oreochromis niloticus)". Dev. Dyn., Vol 237, 297-306.
9. Krisfalljsi M. , Cloud J.G. (1996) "Effects of exogenous estradiol-17β on early growth and gonadal development of diploid and triploid female rainbow trout (Oncorhyncus mykiss)". Dev. Genet., Vol 19, 302-308.<302::AID-DVG3>3.0.CO;2-D
10. Kroon F.J. , Munday P.L. , Pankhurst N.W. (2003) "Steroid hormone levels and bidirectional sex change in Gobiodon histrio ". J. Fish Biol., Vol 62, 153-167.
11. Li M. , Wang L. , Wang H. , Liang H. , Zheng Y. , Qin F. , Liu S. , Zhang Y. , Wang Z. (2013a) "Molecular cloning and characterization of amh, dax1 and cyp19a1a genes in pengze crucian carp and their expression patterns after 17α-methyltestosterone exposure in juveniles". Comp. Biochem. Phys. C, Vol 157, 372-381.
12. Livak K.J. , Schmittgen T.D. (2001) "Analysis of relative gene expression data using real-time quantitative PCR and the 2 − Δ Δ C T method". Methods, Vol 25, 402-408.
13. Luckenbach J.A. , Dickey J.T. , Swanson P. (2011) "Follicle-stimulating hormone regulation of ovarian transcripts for steroidogenesis-related proteins and cell survival, growth and differentiation factors in vitro during early secondary oocyte growth in coho salmon". Gen. Comp. Endocrinol., Vol 171, 52-63.
14. Maruska K.P. , Fernald R.D. (2010) "Steroid receptor expression in the fish inner ear varies with sex, social status, and reproductive state". BMC Neurosci., Vol 11, 58.
15. Mazzeo I. , Peñaranda D.S. , Gallego V. , Hildahl J. , Nourizadeh-Lillabadi R. , Asturiano J.F. , Pérez L. , Weltzien F.A. (2012) "Variations in the gene expression of zona pellucida proteins, zpb and zpc, in female European eel (Anguilla anguilla) during induced sexual maturation". Gen. Comp. Endocrinol., Vol 178, 338-346.
16. Mold D.E. , Kim I.F. , Tsai C.M. , Lee D. , Chang C.Y. , Huang R.C.C. (2001) "Cluster of genes encoding the major egg envelope protein of zebrafish". Mol. Reprod. Dev., Vol 58, 4-14.<4::AID-MRD2>3.0.CO;2-P
17. Mommsen S.P. , Walsh K.L. (1988) "Vitellogenesis and oocyte assembly". In: Hoar W.S. , Randall D.J. (Eds) The Physiology of Developing Fish, Vol vol. XIA, pp.  347-401. Academic Press, New York.
18. Nagahama Y. (2000) "Gonadal steroid hormones: major regulators of gonadal sex differentiation and gametogenesis in fish". In: Norberg B. , Kjesbu O.S. , Taranger G.L. , Andersson E. , Stefansson S.O. (Eds) Proceedings of the 6th International Symposium on Reproductive Physiology of Fish, pp.  211-222. Institute of Marine Research and University of Bergen, Bergen, Norway.
19. Nakamoto M. , Matsuda M. , Wang D.S. , Nagahama Y. , Shibata N. (2006) "Molecular cloning and analysis of gonadal expression of Foxl2 in the medaka, Oryzias latipes ". Biochem. Bioph. Res. Co., Vol 344, 353-361.
20. Nakamoto M. , Wang D.S. , Suzuki A. , Matsuda M. , Nagahama Y. , Shibata N. (2007) "Dax1 suppresses P450arom expression in medaka ovarian follicles". Mol. Reprod. Dev., Vol 74, 1239-1246.
21. Peruzzi S. , Chatain B. , Saillant E. , Haffray P. , Menu B. , Falguière J.C. (2004) "Production of meiotic gynogenetic and triploid sea bass, Dicentrarchus labrax L. 1. Performances, maturation and carcass quality". Aquaculture, Vol 230, 41-64.
22. Rodríguez-Marí A. , Yan Y.L. , Bremiller R.A. , Wilson C. , Cañestro C. , Postlethwait J.H. (2005) "Characterization and expression pattern of zebrafish anti-Müllerian hormone (Amh) relative to sox9a, sox9b, and cyp19a1a, during gonad development". Gene Expr. Patt., Vol 5, 655-667.
23. Sabet S.S. , Imanpoor M.R. , Fatideh B.A. (2009) "Relation between gonadal hormones and sexual maturity of female Kutum Rutilus frisii kutum during spawning season". J. Cell Mol. Res., Vol 1, 97-105.
24. Schultz R.J. (1967) "Gynogenesis and triploidy in the viviparous fish Poeciliopsis ". Science, Vol 157, 1564-1567.
25. Shalaby F.M. , Migeed H.A.E. (2012) "Impact of environmental contaminants on the testes of Oreochromis niloticus with special reference to ultrastructure of spermatozoa in Lake Manzala (Egypt)". J. Environ. Anal. Toxicol., Vol 2, 1-8.
26. Sun P. , You F. , Liu M. , Wu Z. , Wen A. , Li J. , Xu Y. , Zhang P. (2010) "Steroid sex hormone dynamics during estradiol-17β induced gonadal differentiation in Paralichthys olivaceus (Teleostei)". Chin. J. Oceanol. Limnol., Vol 28, 254-259.
27. von Hofsten J. , Larsson A. , Olsson P.E. (2005) "Novel steroidogenic factor-1 homolog (ff1d) is coexpressed with anti-Mullerian hormone (AMH) in zebrafish". Dev. Dyn., Vol 233, 595-604.
28. Wang H. , Tan J.T. , Emelyanov A. , Korzh V. , Gong Z. (2005) "Hepatic and extrahepatic expression of vitellogenin genes in the zebrafish, Danio rerio ". Gene, Vol 356, 91-100.
29. Wang L. , You F. , Weng S. , Wen A. , Wu Z. , Zou Y. , Xin M. , Zhang P. (2015) "Molecular cloning and sexually dimorphic expression patterns of nr0b1 and nr5a2 in olive flounder, Paralichthys olivaceus ". Dev. Genes Evol., Vol 225, 95-104.
30. Wang Y. , Lou S. (2006) "Structural and expression analysis of hepatic vitellogenin gene during ovarian maturation in Anguilla japonica ". J. Steroid Biochem. Mol. Biol., Vol 100, 193-201.
31. Xiao J. , Zou T. , Chen Y. , Chen L. , Liu S. , Tao M. , Zhang C. , Zhao R. , Zhou Y. , Long Y. , You C. , Yan J. , Liu Y. (2011) "Coexistence of diploid, triploid and tetraploid crucian carp (Carassius auratus) in natural waters". BMC Genet., Vol 2, 20.
32. Yang R. , Li B. , Feng H. , Yang C. , Sun Y. , Zhang X. , Luo C. (2003) "Cytogenetic analysis of chromosome number and ploidy of Carassius auratus variety Pengze". Acta Zool. Sinica., Vol 49, 104-109.
33. Zhang S. , Wang S. , Li H. , Li L. (2011) "Vitellogenin, a multivalent sensor and an antimicrobial effector". Int. J. Biochem. Cell Biol., Vol 43, 303-305.
34. Zhao J. , Chen X. (2003) "Phenotypic differentiation of offspring developed from pengze crucian carp eggs activated by heterlogous sperm". Zool. Res., Vol 24, 297-301.
35. Zheng Y. , Liang H. , Xu P. , Li M. , Wang Z. (2014) "Molecular cloning of Pcc-dmrt1s and their specific expression patterns in Pengze crucian carp (Carassius auratus var. Pengze) affected by 17α-methyltestosterone". Fish Physiol. Biochem., Vol 40, 1141-1155.
36. Zheng Y. , Wang L. , Li M. , Liang H. , Qin F. , Liu S. , Wang H. , Wu T. , Zhang Y. , Wang Z. (2013) "Molecular characterization of five steroid receptors from pengze crucian carp and their expression profiles of juveniles in response to 17α-ethinylestradiol and 17α-methyltestosterone". Gen. Comp. Endocrinol., Vol 191, 113-122.

Article metrics loading...



Can't access your account?
  • Tools

  • Add to Favorites
  • Printable version
  • Email this page
  • Subscribe to ToC alert
  • Get permissions
  • Recommend to your library

    You must fill out fields marked with: *

    Librarian details
    Your details
    Why are you recommending this title?
    Select reason:
    Animal Biology — Recommend this title to your library
  • Export citations
  • Key

  • Full access
  • Open Access
  • Partial/No accessInformation