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Effects of hypoxia and dietary vitamin E on growth performance and oxidative status of Cirrhinus mrigala (Ham., 1822)

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Vitamin E is of importance for several physiological processes, some of which also apply to fish. Here, we conducted an experiment to assess the effect of environmental hypoxia and dietary vitamin E on oxidative status and tissue injury in a bottom dwelling carp, Cirrhinus mrigala (Ham., 1822). The four treatments combined oxygen availability (Normoxia/Hypoxua) and Vitamin E presence/absence. Lipid peroxidation parameters such as thiobarbituric acid reactive substances (TBAR), peroxide value (PV), polyunsaturated fatty acids/saturated fatty acid (PUFA/SFA) ratio, catalase (CAT), superoxide dismutase (SOD) and erythrocyte fragility were assessed. The results indicate that exposure to hypoxia elevates these parameters. However, the supplementation of vitamin E via the diet effectively reduced erythrocyte membrane damage (EF) and myeloperoxidase activity (MPO), which were enhanced by the exposure to hypoxia. Dietary vitamin E also improved antioxidant enzyme status in the hypoxia exposed fish, as indicated by the decreased catalase and superoxide dismutase activities. Vitamin E supplementation also compensated for increased levels of peroxide value, thiobarbituric acid reactive substances, and an increased polyunsaturated fatty acids/saturated fatty acid ratio resulting from the exposure to hypoxia. Overall, it can be concluded that fortification of vitamin E in the diet of this carp species, and possibly other fish that live under hypoxic conditions, can restore the antioxidant status and well-being to some extent.

Affiliations: 1: 1Division of Fish Nutrition, Biochemistry and Physiology, ICAR-Central Institute of Fisheries Education, Mumbai-400061, India ; 2: 2Feed Research Laboratory, ICAR-Central Inland Fisheries Research Institute, Barrackpore, Kolkata-700120, India

*Corresponding author; e-mail:

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1. Carbonell T., Rama N., Ramon T. (2009) "Respiratory hypoxia and oxidative stress in the brain, is the endogenous erythropoietin an antioxidant?" Cur. Chem. Biol., Vol 15, 238-252.
2. Chandel N.S., Schumacker P.T. (2000) "Cellular oxygen sensing by mitochondria: old questions, new insight". J. Appl. Physiol., Vol 88, 1880-1889. [Crossref]
3. Clayton D.A. (1993) "Mudskippers". In: Ansell A.D., Gibson R.N., Barnes M. (Eds) Oceanography and Marine Biology: an Annual Review, pp.  507-577. University College London Press, London.
4. Cooper R.U., Clough L.M., Farwell M.A., West T.L. (2002) "Hypoxia-induced metabolic and antioxidant enzymatic activities in the estuarine fish, Leiostomus xanthurus". J. Exp. Mar. Biol., Vol 279, 1-20. [Crossref]
5. Davis K.B., Simco B.A., Li M., Robinson E. (1998) "Effect of reduction of supplementary dietary vitamins on the stress response of channel catfish Ictalurus punctatus". J. Wor. A., Soc., Vol 29, 319-324. [Crossref]
6. Dhaliwal H., Kirshenbaum A., Randhawa A.K., Singal P.K. (1991) "Correlation between antioxidant changes during hypoxia & recovery on reoxygenation". Am. J. Physiol., Vol 261, 632-638.
7. Diaz R.J., Rosenberg R. (1995) "Marine benthic hypoxia: a review of its ecological effects and the behavioural responses of benthic macrofauna". Oceanogr Mar Biol. Annu. Rev., Vol 33, 245-303.
8. Draper H.H., Csallany A.S. (1969) "A simplified haemolysis test for vitamin E deficiency". J. Nutr., Vol 98, 390-394.
9. Farrell O.S., Jackson M.J. (1997) "Dietary polyunsaturated fatty acids, vitamin E & hypoxia/reoxygenation-induced damage to cardiac tissue". Clinic. Chimic. Acta., Vol 267, 197-211. [Crossref]
10. Friesen C.N., Horth N.A., Chapman L.J. (2012) "The effect of hypoxia on sex hormones in an African cichlid Pseudocrenilabrus multicolor victoriae". Comp. Biochem. Physiol. (A), Vol 162, 22-30. [Crossref]
11. Gatlin J.M. (2008) "The growing problem of aquatic hypoxia". Science, Vol 321, 926-929. [Crossref]
12. Heath A.G. (1995) Water Pollution and Fish Physiology. CRC Press, Florida, 359 pp.
13. Henrique M.M.F., Gomes E.F., Coustans M.F., Oliva-Teles A., Davies S.J. (1998) "Influence of practical diets with vitamin C on growth and response to hypoxic-stress of Seabream, Sparus aurata". Aquaculture, Vol 161, 415-426. [Crossref]
14. Ilavazhagan G., Bansal A., Prasad D., Thomas P., Sharma S.K., Kain A.K., Kumar D., Selvamurthy W. (2001) "Effect of vitamin E supplementation on hypoxia-induced oxidative damage in male albino rats". Avia. Space Environ. Med., Vol 72, 899-903.
15. Inan C., Kilic I., Kilic K., Kalayci O., Kotiloglu E. (1995) "The effect of high dose antenatal vitamin E on hypoxia-induced changes in newborn rats". Pediatr. Res., Vol 38, 685-689. [Crossref]
16. Jobling M., Davies P.S. (1980) "Effects of feeding on metabolic rate, and the specific dynamic action in plaice, Pleuronectes platessa". J. Fish Biol., Vol 16, 629-638. [Crossref]
17. Kang M.J., Shin M.S., Park J.N., Lee S.S. (2005) "The effects of polyunsaturated: saturated fatty acids ratios and peroxidisability index values of dietary fats on serum lipid profiles and hepatic enzyme activities in rats". B. J. Nutr., Vol 94(4), 526-532. [Crossref]
18. Keleştemur G.T. (2012) "The antioxidant vitamin (A, C, E) and the lipid peroxidation levels in some tissues of juvenile rainbow trout (Oncorhynchus mykiss, W. 1792) at different oxygen levels". Iran. J. Fish. Sc., Vol 11(2), 315-324.
19. Keleştemur G.T., Özdemir Y. (2013) "Effects of dietary vitamin a and e on growth performance and antioxidant status in blood of juvenile rainbow trout (Oncorhynchus mykiss, w. 1792) exposed to flow rate stress". J. Anim. Plan. Sc., Vol 23(3), 821-827.
20. Keleştemur T., Seven İ. (2013) "Effects of dietary propolis and vitamin E on growth performance and antioxidant status in juvenile rainbow trout (Oncorhynchus mykiss) under different flow rate". Aquacult. Res., Vol 44(7), 1120-1131. [Crossref]
21. Keleştemur T., Tatlı Seven P., Yılmaz S., Tuna Keleştemur G., Pınar S.T.S. (2012) "Effects of dietary propolis and vitamin E on growth performance and antioxidant status in blood of juvenıle rainbow trout (Oncorhynchus mykiss) under different flow rates". Zoologia, Vol 29(2), 99-108.
22. Kraus A., Roth H.P., Kirchgessner M. (1997) "Influence of antioxidants (vitamin C, vitamin E & β-carotene) on the osmotic fragility & components of the primary antioxidant system of erythrocytes in zinc-deficient rats". Trace Elem. Elec., Vol 14, 30-37.
23. Lebold K.M., Jump D.B., Miller G.W., Wright C.L., Labut E.M., Barton C.L., Tanguay R.L., Traber M.G. (2011) "Vitamin E deficiency decreases long-chain PUFA in zebrafish (Danio rerio)". J. Nutr., Vol 141, 2113-2118. [Crossref]
24. Liu B., Xu P., Xie J., Ge X., Xia S., Song C. , et al (2014) "Effects of emodin and vitamin E on the growth and crowding stress of Wuchang bream (Megalobrama amblycephala)". Fish. Shellfish. Immunol., Vol 40, 595-602. [Crossref]
25. Lushchak V.I., Bagnyukova T. (2006) "Temperature increase results in oxidative stress in goldfish tissues. Antioxidant and associated enzymes". Comp. Biochem. Physiol., Vol 143, 36-41.
26. Lushchak V.I., Lushchak L.P., Mota A.A., Hermes-Lima M. (2001) "Oxidative stress and antioxidant defenses in goldfish Carassius auratus during anoxia and reoxygenation". Am. J. Physiol., Vol 280, 100-107.
27. Maiti P., Sharma S.B., Muthuraju A.K., Banerjee S., Ilavazhagan G. (2006) "Hypobaric hypoxia induces oxidative stress in rat brain". Neurochem. Int., Vol 49, 709-716. [Crossref]
28. Misra H.P., Fridovich I. (1972) "The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase". J. Biol. Chem., Vol 247(10), 170-175.
29. Moller P., Loft S., Lundby C., Olsen N.V. (2001) "Acute hypoxia & hypoxic exercise induce DNA strand breaks and oxidative DNA damage in humans". FASEB J., Vol 15, 1181-1186. [Crossref]
30. Nakanishi K., Tajima F., Nakamura A., Yagura S., Ookawara T., Yamashita H., Suzuki K., Taniguchi N., Ohno H. (1995) "Effects of hypobaric hypoxia on antioxidant enzymes in rats". J. Physiol., Vol 489, 869-876. [Crossref]
31. NRC (National Research Council) (2011) Nutrient Requirement of Fish and Shrimp. National Academy Press, Washington, DC, USA, 376 pp.
32. Ohkawa H., Ohishi N., Yagi K. (1979) "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction". Anal. Biochem., Vol 95, 357-358. [Crossref]
33. Ortuno J., Esteban M.A., Meseguer H. (2003) "The effect of dietary intake of vitamins C & E on the stress response of gilthead seabream (Sparus aurata)". Fish Shellfish. Immunol., Vol 14, 145-156. [Crossref]
34. Otamiri T., Lindahl M., Tagesson C. (1988) "Phospholipase A2 inhibition prevents mucosal damage associated with small intestinal ischemia in rats". Gut, Vol 29, 489-494. [Crossref]
35. Pattison D.I., Davies M.J. (2006) "Reactions of myeloperoxidase-derived oxidants with biological substrates: gaining chemical insight into human inflammatory diseases". Curr. Med. Chem., Vol 13(27), 3271-3290. [Crossref]
36. Quade M.J., Roth J.A. (1997) "A rapid direct assay to measure degranulation of bovine neutrophil primary granular". Vet. Immunol. Immunopathol., Vol 58, 339-348. [Crossref]
37. Randall D.J., Mckenzie G., Abrami G.P., Bondiolotti F., Natiello P., Bronzi L. (1992) "Effects of diet on responses to hypoxia in sturgeon (Acipenser naccarit)". J. Exp. Biol., Vol 170, 113-125.
38. Sahoo P.K., Jaya K., Mishra B.K. (2005) "Non-specific immune responses in juveniles of Indian major carps". J. Appl. Ichthyol., Vol 21, 151-155. [Crossref]
39. Saiki T. (1998) "Myeloperoxidase concentrations in the stool as a new parameter of inflammatory bowel disease". Kurume. Med. J., Vol 45, 69-73. [Crossref]
40. Sairam R.K., Kumutha D., Ezhilmathi K., Deshmukh P.S., Srivastava G.C. (2008) "Physiology and biochemistry of waterlogging tolerance in plants". Biol. Plan., Vol 52, 401-412. [Crossref]
41. Sealey W.M., Gatlin D.M. (2002) "Dietary vitamin C & vitamin E interact to influence growth & tissue composition of juvenile hybrid striped bass but have limited effects on immune responses". J. nutr., Vol 132, 748-755.
42. Shantha N.C., Decker E.A. (1994) "Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids". J. AOAC Int., Vol 77, 421-424.
43. Sharma B., Rai D.K., Rai P.K., Rizvi S.I., Watal G. (2010) "Determination of erythrocyte fragility as a marker of pesticide-induced membrane oxidative damage". Method. Mol. Biol., Vol 594, 123-128. [Crossref]
44. Suarez A., Ramirez-Tortosa M., Gil A., Faus M. (1999) "Addition of vitamin-E to long-chain polyunsaturated fatty acid-enriched diets protects neonatal tissue lipids against peroxidation in rats". Eur. J. Nutr., Vol 38(4), 169-176. [Crossref]
45. Takahara S., Hamilton H.B., Neel J.V., Kobara T.Y., Ogura Y., Nishimura E.T. (1960) "Hypocatalasemia, a new genetic carrier state". J. Clin. Invest., Vol 39, 610-619. [Crossref]
46. Winston G.W., Di Guilio R.T. (1991) "Prooxidant and antioxidant mechanisms in aquatic organisms". Aqua. Toxicol., Vol 19, 137-161. [Crossref]
47. Winterbourn C.C., Hampton M.B., Livesey J.H., Kettle A.J. (2006) "Modeling the reactions of superoxide and myeloperoxidase in the neutrophil phagosome: implications for microbial killing". J. Biol. Chem., Vol 281(52), 39860-39869. [Crossref]

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