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A Rapid Method for the Transfer of Nematodes From Fixative To Anhydrous Glycerin

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image of Nematologica
For more content, see Nematology.

Eine Methode wird beschrieben bei der Nematoden innerhalb 24 Stunden von der Fixierungsflüssigkeit in wasserfreies Glyzerin überführt werden. Die Nematoden werden nach Fixierung in ein Gemisch von 20 Teilen Aethanol 96%, I Teil Glyzerin und 79 Teilen Wasser gebracht. Das Aethanol in diesem Gemisch wird durch Wasserentzug im Exsiccator mit Aethanol 96% bei 35°-40°C in nicht weniger als 12 Stunden bis zu etwa 95% konzentriert. Danach wird ein Gemisch von 5 Teilen Glyzerin in 95 Teilen Aethanol 96% hinzugefügt. Dieser wird bei 40°C in wenigstens 3 Stunden zu reines Glyzerin konzentriert.

Affiliations: 1: Instituut voor Plantenziektenkundig Onderzoek, Wageningen, Netherlands


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