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Recovery of Nematodes From Infected Roots By Maceration

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image of Nematologica
For more content, see Nematology.

A procedure for the recovery of quantities of living root-knot nematodes (Meloidogyne spp.) from galled roots has been developed. Infected roots are macerated by pectolytic enzymes obtained commercially or prepared from potatoes inoculated with Erwinia carolovora, Roots are immersed in enzymes in flasks at room temperatures with constant agitation in a wrist-action shaker. Chemical agents (periodic acid, or a mixture of chromic and nitric acids) also macerate infected roots; the nematodes are killed but their bodies remain intact. Nematodes are separated from plant debris by washing with tap water through a nest of sieves, followed by decantation. Giant cells also may be recovered on screens. The procedure should prove useful in obtaining live endoparasitic nematodes in quantity for physiological and biochemical studies, for estimating the population in infected roots, and for the study of pathological cell types.

Affiliations: 1: Crops Research Division, Agricultural Research Service; 2: Market Quality Research Division, Agricultural Marketing Service; 3: United States Department of Agriculture, Beltsville, Maryland, U.S.A.


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