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Cyst batches of approximately equal size for use in bioassays can be set up with a simple pipette and the larvae that hatch from them can be assessed by absorptiometry, which saves the time and tedium of counting both cysts and larvae. In an experiment on the use of charcoal to recover the hatching factor from potato root diffusate, the results of bioassays by this method agreed well with those based on counts. The interpretation of data is discussed.