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Caenorhabditis Briggsae (Rhabditidae) Under Anaerobic Conditions

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The effect of anaerobic conditions on the eggs, larval stages and adults of C. briggsae was investigated. Anaerobic conditions were produced by exposing the eelworms, in drops of media, to a stream of carefully purified moist N2 or H2. Temperature, the osmotic properties of the media in which the worms were suspended, and pH were considered in designing the experiments. The nematodes were maintained under axenic conditions while anaerobic. It is concluded that swimming is impaired by O2 lack, rapidly leading to complete paralysis. Eelworms recover when returned to aerobic conditions after a short period of paralysis, but after longer periods, of the order of 24 hours, they fail to recover. Eggs survive for several days under anaerobic conditions, but hatching is inhibited until conditions become aerobic.

Affiliations: 1: Department of Zoology, Australian National University, Canberra

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/content/journals/10.1163/187529264x00420
1964-01-01
2016-12-09

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