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Methods for the Study of Plant Parasitic Nematodes in Gnotobiotic Root Culture

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image of Nematologica
For more content, see Nematology.

Two types of apparatus were evaluated for use in the study of plant-nematode interrelationships in gnotobiotic culture. The smaller and less complicated of these apparatus proved the more successful. Two plant species were grown for periods occasionally exceeding 3 months, and several nematode species increased rapidly under the defined experimental environment. Over 55 % of the smaller units were uncontaminated at the conclusion of the test period. Symptoms associated with the feeding of Meloidogyne hapla developed rapidly. Host reactions to feeding of ectoparasitic nematodes were not evaluated.

Affiliations: 1: Cranberry Experiment Station, University of Massachusetts, East Wareham, Mass., U.S.A.


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