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Staining of Free-Living Nematodes By Eosin-y dYe

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For more content, see Nematology.

A staining technique has been developed in conjunction with the authors' studies of free-living nematodes in water supplies. It employs eosin-Y dye which stains dead nematodes, while live nematodes remain unstained. The procedure also assists in counting nematodes and does not interfere with subsequent basic taxonomic identification. A short staining period is employed to facilitate routine examinations and to minimize changes in the samples. A dye concentraton which does not immobilize living nematodes during the staining period has been established. Several representative lethal agents have been employed to evaluate the versatility of the staining procedure, and the systematic error inherent in the technique has been evaluated.

Affiliations: 1: Department of Civil Engineering, University of Illinois, Urbana, Illinois, U.S.A.


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