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Tissue Culture and Maintenance of the Root-Lesion Nematode, Pratylenchus Vulnus

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For more content, see Nematology.

A mixture of callus and differentiated alfalfa tissue grown on a nutrient agar provides a favorable medium for the monoxenic culture of Pratylenchus vulnus. The nematode increases more rapidly at 25° C than at 20°, and does not increase at 30° or 35°. At 25°, high populations of P. vulnus can be obtained 2 months after inoculation of the cultures. These high populations can be maintained longer at 15° or 10° than at 5°, 20°, or 25°. Nematode-free alfalfa tissue grew well over the range 20° to 30°, poorly at 35°. P. vulnus reduced the fresh weight of alfalfa callus at 20° and 25°, the temperatures favorable for nematode reproduction.

Affiliations: 1: Department of Nematology, University of California, Davis, U.S.A


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